医药前沿
醫藥前沿
의약전연
YIAYAO QIANYAN
2015年
22期
25-25,26
,共2页
巩凡%程锁利%吕强%王立峰%魏松英
鞏凡%程鎖利%呂彊%王立峰%魏鬆英
공범%정쇄리%려강%왕립봉%위송영
激素性股骨头坏死%PPARγ%SREBP-1c
激素性股骨頭壞死%PPARγ%SREBP-1c
격소성고골두배사%PPARγ%SREBP-1c
Sanfh%PPARγ%SREBP-1c
目的:观察兔激素性股骨头缺血性坏死骨质中SREBP-1c及PPARγ的表达。方法:使用醋酸泼尼松龙制备兔激素性股骨头缺血性坏死动物模型,采用实时荧光定量PCR 检测PPARγ、SREBP-1c在骨组织中的表达情况。结果:PPARγ、SREBP-1c的表达量在骨组织中较正常组织中显著增多(P<0.05);实验组中PPARγ表达量比SREBP-1c mRNA 显著增多(P<0.05)。结论:SREBP-1c及PPARγ的信号转导途径影响脂肪细胞的分化,其在兔激素性股骨头坏死脂质代谢中发挥重要的作用。
目的:觀察兔激素性股骨頭缺血性壞死骨質中SREBP-1c及PPARγ的錶達。方法:使用醋痠潑尼鬆龍製備兔激素性股骨頭缺血性壞死動物模型,採用實時熒光定量PCR 檢測PPARγ、SREBP-1c在骨組織中的錶達情況。結果:PPARγ、SREBP-1c的錶達量在骨組織中較正常組織中顯著增多(P<0.05);實驗組中PPARγ錶達量比SREBP-1c mRNA 顯著增多(P<0.05)。結論:SREBP-1c及PPARγ的信號轉導途徑影響脂肪細胞的分化,其在兔激素性股骨頭壞死脂質代謝中髮揮重要的作用。
목적:관찰토격소성고골두결혈성배사골질중SREBP-1c급PPARγ적표체。방법:사용작산발니송룡제비토격소성고골두결혈성배사동물모형,채용실시형광정량PCR 검측PPARγ、SREBP-1c재골조직중적표체정황。결과:PPARγ、SREBP-1c적표체량재골조직중교정상조직중현저증다(P<0.05);실험조중PPARγ표체량비SREBP-1c mRNA 현저증다(P<0.05)。결론:SREBP-1c급PPARγ적신호전도도경영향지방세포적분화,기재토격소성고골두배사지질대사중발휘중요적작용。
Objective To observe the expression of SREBP-1 c and PPARγin Rabbit glucocorticoid-induced avascular necrosis. Methods Prednisolone acetate was used to establish the animal model of rabbit glucocorticoid-induced avascular necrosis, and the expression of SREBP-1 c and PPARγin bone tissue were detected by real-time fluorescent quantitative PCR assay. Results The expression of PPARγmRNA in bone tissue were higher than normal tissue (P<0.05);The expression of PPARγwas higher than in the SREBP-1c in the experimental group (P<0.05). Conclusion Signal transduction pathway of SREBP-1c and PPARγplay an important role in glucocorticoid induced avascular necrosis lipid metabolism by effecting fat differentiation.