海峡药学
海峽藥學
해협약학
STRAIT PHARMACEUTICAL JOURNAL
2015年
8期
26-28
,共3页
桔梗%蜜炙桔梗%指纹图谱%桔梗皂苷D
桔梗%蜜炙桔梗%指紋圖譜%桔梗皂苷D
길경%밀자길경%지문도보%길경조감D
Radix Platycodonis%Radix Platycodonis Preparatus cum Melle%Fingerprint%Platycodin-D
目的：比较桔梗蜜炙前后饮片高效液相指纹图谱。方法采用Agilent C18色谱柱（4．6mm ×250mm，5μm），流动相为0．1％磷酸水溶液－乙腈，梯度洗脱，检测波长210nm，流速1．0mL? min －1。计算各被测样品的 HPLC指纹图谱的整体相似度。结果分别建立了桔梗、蜜炙桔梗饮片的指纹图谱，确定了20个共有峰，确认了桔梗皂苷D色谱峰。结论该法简便、快捷、可靠，可为桔梗、蜜炙桔梗饮片质量标准的建立提供一定的参考依据。
目的：比較桔梗蜜炙前後飲片高效液相指紋圖譜。方法採用Agilent C18色譜柱（4．6mm ×250mm，5μm），流動相為0．1％燐痠水溶液－乙腈，梯度洗脫，檢測波長210nm，流速1．0mL? min －1。計算各被測樣品的 HPLC指紋圖譜的整體相似度。結果分彆建立瞭桔梗、蜜炙桔梗飲片的指紋圖譜，確定瞭20箇共有峰，確認瞭桔梗皂苷D色譜峰。結論該法簡便、快捷、可靠，可為桔梗、蜜炙桔梗飲片質量標準的建立提供一定的參攷依據。
목적：비교길경밀자전후음편고효액상지문도보。방법채용Agilent C18색보주（4．6mm ×250mm，5μm），류동상위0．1％린산수용액－을정，제도세탈，검측파장210nm，류속1．0mL? min －1。계산각피측양품적 HPLC지문도보적정체상사도。결과분별건립료길경、밀자길경음편적지문도보，학정료20개공유봉，학인료길경조감D색보봉。결론해법간편、쾌첩、가고，가위길경、밀자길경음편질량표준적건립제공일정적삼고의거。
OBJECTIVE To compare the fingerprint of HPLC for Radix Platycodonis before and after stir-fried with honey.METHODS The column of AGILENT C18 (4.6mm ×250mm,5μm) was used.The mobile phase con-sisted of 0.1%Orthophosphoric acid and acetonitrile,with gradient elution.The detective wavelength was at 210nm, and the flow rate was 1.0 mL? min-1 .The similarity of fingerprint of each sample was calculated.RESULTS Es-tablishing the fingerprint of consisted of Radix Platycodonis before and after stir-fried with honey,20 common peaks were found in the HPLC fingerprint from different samples,and the platycodin-D was conformed.CONCLUSION This method was rapid, simple, accurate and reliable and provide a scientific basis of the quality control of Radix Platycodonis before and after processing.
