食品研究与开发
食品研究與開髮
식품연구여개발
FOOD RESEARCH AND CEVELOPMENT
2015年
16期
142-144
,共3页
何义%朱景松%姜旋%苑宁%张伟
何義%硃景鬆%薑鏇%苑寧%張偉
하의%주경송%강선%원저%장위
八宝粥%软罐头%致腐菌%鉴定
八寶粥%軟罐頭%緻腐菌%鑒定
팔보죽%연관두%치부균%감정
rice porridge%soft can%rot fungus%identification
从胀袋的八宝粥软罐头中分离出一株致腐细菌zjs001，对其进行形态观察、革兰氏染色和API 20E生化鉴定，同时提取其基因组DNA，进行16SrDNA PCR扩增，对扩增产物进行测序，与NCBI中已知序列进行比对，以确定其种属，研究结果表明，引起八宝粥胀袋的腐败菌为阴沟肠杆菌。
從脹袋的八寶粥軟罐頭中分離齣一株緻腐細菌zjs001，對其進行形態觀察、革蘭氏染色和API 20E生化鑒定，同時提取其基因組DNA，進行16SrDNA PCR擴增，對擴增產物進行測序，與NCBI中已知序列進行比對，以確定其種屬，研究結果錶明，引起八寶粥脹袋的腐敗菌為陰溝腸桿菌。
종창대적팔보죽연관두중분리출일주치부세균zjs001，대기진행형태관찰、혁란씨염색화API 20E생화감정，동시제취기기인조DNA，진행16SrDNA PCR확증，대확증산물진행측서，여NCBI중이지서렬진행비대，이학정기충속，연구결과표명，인기팔보죽창대적부패균위음구장간균。
A strain of pathogenic Microbal zjs001 was successfully isolated from the spoilage of one soft can of rice pudding, by morphological observation. Gram staining and biochemical characterization of 20E API were performed, and the genomic DNA was extracted, and PCR 16SrDNA was amplified and sequenced. The amplified products were sequenced and compared with the known sequences in NCBI ,identified as Enterobacter cloacae.
