CAJ | 학술논문

从储藏玉米中分离纯化得到霉菌的单菌落，并对菌种进行鉴定。对玉米进行5次取样，分离到60个单菌落，其中曲霉属检出率最高，青霉属次之。采用点植法对霉菌在察氏培养基和高盐察氏培养基上的生长情况，包括菌落直径、质地、颜色、渗出物有无等进行记录，同时在光学显微镜下对菌体结构进行观察，参照菌落标准图谱，鉴定出5种霉菌，分别为黑曲霉、烟曲霉、产黄青霉、桔青霉和缓生青霉，为今后玉米储藏中的菌相研究提供参考。
종저장옥미중분리순화득도매균적단균락，병대균충진행감정。대옥미진행5차취양，분리도60개단균락，기중곡매속검출솔최고，청매속차지。채용점식법대매균재찰씨배양기화고염찰씨배양기상적생장정황，포괄균락직경、질지、안색、삼출물유무등진행기록，동시재광학현미경하대균체결구진행관찰，삼조균락표준도보，감정출5충매균，분별위흑곡매、연곡매、산황청매、길청매화완생청매，위금후옥미저장중적균상연구제공삼고。
Molds derived from corn were separated and purified by single colony , and identified. Corn samples were taken for 5 times from stored corn , and 60 single colonies were isolated. The highest detection rate was of Aspergillus, the higher was Penicillium. The growth situations of molds from corn were recorded, which included colony's diameter, texture, color, and exudates presence of molds in czapek's medium and high salt czapek's medium separately. And the microscopic morphology of molds from corn were observed by optical microscope. Compared with the standard figures , five kinds of molds from corn were identified as follows:Aspergillus funigatus, Aspergillus niger, Penicillium chrysogenu, Penicillium citrinum and Penicillium tardum. This result will provide reference to study further microflora in corn.