CAJ | 학술논문

目的：从分子水平探讨姜黄素的降脂机制，为姜黄素作为降脂药物的临床开发提供科学依据。方法本研究运用油红 O 染色、酶法测定细胞内胆固醇含量，荧光染色检测细胞胆固醇摄取，RT-Q-PCR 及 Western blot 检测姜黄素对HepG2细胞内胆固醇代谢相关因子在 RNA 和蛋白水平的表达。结果姜黄素组的 HepG2细胞内红色脂滴明显增多，且TC 及 FC 含量增高。DiI 标记 LDL，姜黄素组 HepG2细胞橙红色荧光高于对照组。姜黄素能升高 SREBP2和 LDLR 的mRNA 和蛋白水平的表达；降低 PCSK9蛋白成熟体及 IDOL蛋白表达。结论姜黄素可能通过下调 PCSK9及 IDOL 的表达，进而减少 LDLR 降解，促进 HepG2细胞摄取 LDL-C。
목적：종분자수평탐토강황소적강지궤제，위강황소작위강지약물적림상개발제공과학의거。방법본연구운용유홍 O 염색、매법측정세포내담고순함량，형광염색검측세포담고순섭취，RT-Q-PCR 급 Western blot 검측강황소대HepG2세포내담고순대사상관인자재 RNA 화단백수평적표체。결과강황소조적 HepG2세포내홍색지적명현증다，차TC 급 FC 함량증고。DiI 표기 LDL，강황소조 HepG2세포등홍색형광고우대조조。강황소능승고 SREBP2화 LDLR 적mRNA 화단백수평적표체；강저 PCSK9단백성숙체급 IDOL단백표체。결론강황소가능통과하조 PCSK9급 IDOL 적표체，진이감소 LDLR 강해，촉진 HepG2세포섭취 LDL-C。
Aim To explore the lipid-lowering mecha-nisms of curcumin from the molecular levels and pro-vide scientific basis for clinical development of lipid-lowering drugs.Methods Using oil red O staining and enzymic to determinate the levels of cholesterol in HepG2 cells.Moreover,uptaking of DiI-LDL was also measured.The expressions of mRNA and protein were detected by RT-Q-PCR and Western blot.Results The red lipid droplets and the levels of TC and FC sig-nificantly increased in HepG2 cells after treated with curcumin.The orange red fluorescence was higher than that of control.Curcumin could promote the expression levels of mRNA and protein of SREBP2 and LDLR, what′s more,curcumin could reduce the expression of the mature PCSK9 level and IDOL protein.Conclu-sion Curcumin accelerates LDL-C uptake probably via downregulating the expression of PCSK9 and IDOL in HepG2 cells.