CAJ | 학술논문

以商品化的红曲米为原料，利用质谱图定性分析，考察在红曲红色素提取过程中冷冻，索氏提取，溶剂，柱色谱，薄层色谱等因素对分离效果的影响，筛选并分离得到一种分子离子峰为332.6红曲红色素分子。研究结果表明该红曲红色素分子的纯化条件依次为：以甲醇为淋洗剂对红曲米进行柱色谱分离，以正己烷、乙酸乙酯和甲醇为萃取剂进行一次索氏提取，以CH2Cl2∶CH3OH=1∶1为展开剂进行薄层色谱分离，收集Rf=0.8的色带，以CH3CH2OH∶CCl4=9∶1为展开剂进行第二次薄层色谱分离，收集Rf=0.8的色带为目标分子，收率为0.0126%。
이상품화적홍곡미위원료，이용질보도정성분석，고찰재홍곡홍색소제취과정중냉동，색씨제취，용제，주색보，박층색보등인소대분리효과적영향，사선병분리득도일충분자리자봉위332.6홍곡홍색소분자。연구결과표명해홍곡홍색소분자적순화조건의차위：이갑순위림세제대홍곡미진행주색보분리，이정기완、을산을지화갑순위췌취제진행일차색씨제취，이CH2Cl2∶CH3OH=1∶1위전개제진행박층색보분리，수집Rf=0.8적색대，이CH3CH2OH∶CCl4=9∶1위전개제진행제이차박층색보분리，수집Rf=0.8적색대위목표분자，수솔위0.0126%。
Commercial monascus red pigments were used as the raw material , the influence of the freezing temperature, Soxhlet extraction, solvents, column chromatography and thin layer chromatography separation process assisted by the use of mass methods were studied in details. A new kind of monasucs was screened out from the raw materials, the molecular ion peak located at 332.5. The experimental conditions for the purification of the monascus was:Before the Soxhlet extraction which hexane , ethyl acetate and methanol as the separated solvents, the red yeast rice subjected to column chromatography by the methanol used as the eluent. Then CH2Cl2∶CH3OH=1∶1 were used as the eluent for the thin layer chromatography ( Rf=0.8). Finally, a second thin layer chromatography were carried out to collect the the target molecule bond (Rf= 0.8) in which the CH3CH2OH∶CCl4=9∶1 were used as the corresponding eluents, yield 0.0126%.