中国药理学通报
中國藥理學通報
중국약이학통보
CHINESE PHARMACOLOGICAL BULLETIN
2015年
9期
1249-1253
,共5页
刘梅%罗海%林嘉伟%魏燕%李媛%刘善文%孟龙%邹丽莉%朱林燕%王立伟%陈丽新
劉梅%囉海%林嘉偉%魏燕%李媛%劉善文%孟龍%鄒麗莉%硃林燕%王立偉%陳麗新
류매%라해%림가위%위연%리원%류선문%맹룡%추려리%주림연%왕립위%진려신
阿霉素%鼻咽肿瘤%氯通道%膜片钳技术%凋亡%凋亡性容积减小
阿黴素%鼻嚥腫瘤%氯通道%膜片鉗技術%凋亡%凋亡性容積減小
아매소%비인종류%록통도%막편겸기술%조망%조망성용적감소
adriamycin%nasopharyngeal neoplasms%chloride channels%patch clamp technique%apoptosis%apoptotic volume decrease
目的：研究氯离子通道在阿霉素诱导的低分化鼻咽癌CNE-2Z 细胞凋亡及凋亡性容积减小中的作用。方法采用全细胞膜片钳技术记录氯电流，流式细胞术检测细胞凋亡，活细胞图像分析法检测细胞容积变化。结果（1）阿霉素诱导 CNE-2Z 细胞凋亡；（2）阿霉素诱导 CNE-2Z 细胞发生凋亡性容积减小，细胞外灌流阿霉素2 h，细胞容积减小约10％；（3）阿霉素可激活氯通道，产生一个有较明显外向优势的氯电流；（4）氯通道阻断剂5-nitro-2-（3-phenylpropylami-no）-benzoate （NPPB）明显抑制阿霉素诱导的凋亡性容积减小、细胞凋亡和氯电流。结论阿霉素可通过激活氯通道而诱导细胞凋亡，氯通道在阿霉素诱导的鼻咽癌细胞凋亡中发挥重要作用。
目的：研究氯離子通道在阿黴素誘導的低分化鼻嚥癌CNE-2Z 細胞凋亡及凋亡性容積減小中的作用。方法採用全細胞膜片鉗技術記錄氯電流，流式細胞術檢測細胞凋亡，活細胞圖像分析法檢測細胞容積變化。結果（1）阿黴素誘導 CNE-2Z 細胞凋亡；（2）阿黴素誘導 CNE-2Z 細胞髮生凋亡性容積減小，細胞外灌流阿黴素2 h，細胞容積減小約10％；（3）阿黴素可激活氯通道，產生一箇有較明顯外嚮優勢的氯電流；（4）氯通道阻斷劑5-nitro-2-（3-phenylpropylami-no）-benzoate （NPPB）明顯抑製阿黴素誘導的凋亡性容積減小、細胞凋亡和氯電流。結論阿黴素可通過激活氯通道而誘導細胞凋亡，氯通道在阿黴素誘導的鼻嚥癌細胞凋亡中髮揮重要作用。
목적：연구록리자통도재아매소유도적저분화비인암CNE-2Z 세포조망급조망성용적감소중적작용。방법채용전세포막편겸기술기록록전류，류식세포술검측세포조망，활세포도상분석법검측세포용적변화。결과（1）아매소유도 CNE-2Z 세포조망；（2）아매소유도 CNE-2Z 세포발생조망성용적감소，세포외관류아매소2 h，세포용적감소약10％；（3）아매소가격활록통도，산생일개유교명현외향우세적록전류；（4）록통도조단제5-nitro-2-（3-phenylpropylami-no）-benzoate （NPPB）명현억제아매소유도적조망성용적감소、세포조망화록전류。결론아매소가통과격활록통도이유도세포조망，록통도재아매소유도적비인암세포조망중발휘중요작용。
Aim To investigate the roles of chloride channels in the apoptosis and apoptotic volume de-crease (AVD)induced by adriamycin in nasopharyn-geal carcinoma CNE-2Z cells.Methods Apoptotic rates were detected by flow cytometry,and the volume changes were measured by the time-lapse live cell ima-ging technique.The patch clamp technique was used to record whole-cell chloride currents.Results Adria-mycin induced apoptosis of CNE-2Z cells.An early ap-optotic volume decrease was observed in the cell trea-ted with adriamycin.The cell volume was decreased by about 10% in 2 h.Adriamycin activated a chloride current which showed outward rectification.The chlo-ride channel blocker 5-nitro-2-(3-phenylpropylamino)-benzoate (NPPB ) could inhibit the adriamycin-in-duced chloride currents,apoptosis and prevent cell shrinkage.Conclusions Our findings suggest that ad-riamycin causes cell apoptosis by activation of chloride channels.Chloride channels may be involved in the apoptosis and apoptotic volume decrease induced by adriamycin in CNE-2Z cells.
