CAJ | 학술논문

探究超声辅助法提取紫花苜蓿多糖的最佳工艺，并对其进行体外抗肿瘤活性研究。利用超声辅助提取法提取紫花苜蓿多糖；利用细胞活力测定法、LDH活性测定和双荧光染色法研究紫花苜蓿多糖的体外抗肿瘤活性。细胞活力分析仪测定结果显示紫花苜蓿多糖能抑制人乳腺癌细胞的活性；LDH活性测定表明紫花苜蓿多糖能影响人乳腺癌细胞并呈现出剂量-效应关系；双荧光染色法结果表明紫花苜蓿多糖作用后人乳腺癌细胞凋亡数量较对照组增加；紫花苜蓿多糖具有较为良好的体外抗肿瘤活性。
탐구초성보조법제취자화목숙다당적최가공예，병대기진행체외항종류활성연구。이용초성보조제취법제취자화목숙다당；이용세포활력측정법、LDH활성측정화쌍형광염색법연구자화목숙다당적체외항종류활성。세포활력분석의측정결과현시자화목숙다당능억제인유선암세포적활성；LDH활성측정표명자화목숙다당능영향인유선암세포병정현출제량-효응관계；쌍형광염색법결과표명자화목숙다당작용후인유선암세포조망수량교대조조증가；자화목숙다당구유교위량호적체외항종류활성。
To discuss the best ultrasonic-assisted extraction condition of the total polysaccharides from Medicago sativaL and to study the antitumor activity in vitro of total polysaccharides from Medicago sativaL. Ultrasonic-assisted extraction was employed in the extraction of the total polysaccharides from Medicago sativaL. Antitumor activity of total polysaccharides from Medicago sativaL was measured by using the cell viability assay, LDH activity and double staining immunofluorescence techniques. Cell viability assay and LDH activity measurement indicated that total polysaccharides from Medicago sativaL can dose-dependently inhibited the proliferation activity of human breast cancer cells. Double staining immunofluorescence techniques showed that human breast cancer cells apparently increased after the treatment of total polysaccharides from Medicago sativaL than the control groups. Total polysaccharides from Medicago sativaL had comparatively obvious antitumor activity in vitro.