CAJ | 학술논문

目的：研究黄芪多糖（AP）对冻融人精子DNA完整性的保护作用。方法：收集正常（7份）和低活力（5份）共12例患者的精液标本，分别用含0，10，25，50 mg/L AP和1 g/L维生素E（VE）的冷冻保护液冻存精液，经过常规冻融和吖啶橙染色后，在荧光显微镜下计算精子的DNA碎片指数（DFI）。结果：在正常精子组中，T1（含10 mg/L AP）样本的DFI值比T0（含0 mg/L AP）、T2（含25 mg/L AP）、T3（含50 mg/L AP）和T4（含1 g/L VE）样本降低（P＜0.05）；在低活力精子组中，T1样本的DFI值低于T0和T4样本，差异有统计学意义（P＜0.05）。结论：AP在适当浓度时（10 mg/L）对冷冻保存的人精子DNA完整性有保护作用。
목적：연구황기다당（AP）대동융인정자DNA완정성적보호작용。방법：수집정상（7빈）화저활력（5빈）공12례환자적정액표본，분별용함0，10，25，50 mg/L AP화1 g/L유생소E（VE）적냉동보호액동존정액，경과상규동융화아정등염색후，재형광현미경하계산정자적DNA쇄편지수（DFI）。결과：재정상정자조중，T1（함10 mg/L AP）양본적DFI치비T0（함0 mg/L AP）、T2（함25 mg/L AP）、T3（함50 mg/L AP）화T4（함1 g/L VE）양본강저（P＜0.05）；재저활력정자조중，T1양본적DFI치저우T0화T4양본，차이유통계학의의（P＜0.05）。결론：AP재괄당농도시（10 mg/L）대냉동보존적인정자DNA완정성유보호작용。
Objective:To examine the protective effect of astragalus polyose (AP) on the DNA integrity of human sperm underwent freezing-thawing. Methods:The human semen samples with normal motility (7 samples) or low motility (5 samples) were collected. After grouped, the sperm suspension were freezed with the cryoprotective medium contained 0, 10, 25 and 50 mg/L of AP (the T0, T1, T2, T3 groups). The positive control is 1 g/L of Vitamin E (the T4 group). After the conventionally freezing-thawing, and dyeing with acridine orange, those sperm samples were detected the DNA fragmentation index (DFI) using fluorescence microscope. Results:In those groups of normal sperm motility, the DFI in the T1 group was significantly lower than those in the T0, T2, T3 and T4 groups (P<0.05). In those groups of low sperm motility, the DFI in the T1 group was significantly lower than those in the T0 group and the T4 group (P<0.05). Conclusions:The AP at 10 mg/L concentration has a protective effect on the DNA integrity of human sperm underwent freezing-thawing.