CAJ | 학술논문

目的建立 UPLC-MS 法测定大鼠口服荭草花提取物后血浆中原儿茶酸、山柰素－葡萄糖、槲皮苷的分析方法，并用该方法研究原儿茶酸等3个指标成分的药物代谢动力学。方法血浆样品选择酸化后甲醇沉淀蛋白，Waters BEH C18（2.1 mm ×100 mm，1.7μm）柱，流速：0.30 mL·min －1，流动相：0.1％甲酸乙腈－0.1％甲酸水梯度洗脱，采用电喷雾电离源（ESI），扫描方式为多反应离子监测（SIR）。结果3种成分分别在2.74～677、0.69～186、0.82～200μg·L －1呈良好线性关系。提取回收率、精密度、准确度和稳定性均良好达到测定要求。原儿茶酸、山柰素－葡萄糖和槲皮苷3个指标成分的 Tmax （h）分别为0.46±0.1、0.79±0.33和2.63±4.6，Cmax （μg·L －1）分别为463.8±207.81、18.53±7.82和137.38±71.09。结论建立的 UPLC-MS 检测方法具有特异、快速、准确和灵敏的特点，能够同时测定生物样品中原儿茶酸等3种成分的血药浓度，并成功应用于荭草花在正常大鼠体内这3个指标成分的药动学研究。
목적건립 UPLC-MS 법측정대서구복홍초화제취물후혈장중원인다산、산내소－포도당、곡피감적분석방법，병용해방법연구원인다산등3개지표성분적약물대사동역학。방법혈장양품선택산화후갑순침정단백，Waters BEH C18（2.1 mm ×100 mm，1.7μm）주，류속：0.30 mL·min －1，류동상：0.1％갑산을정－0.1％갑산수제도세탈，채용전분무전리원（ESI），소묘방식위다반응리자감측（SIR）。결과3충성분분별재2.74～677、0.69～186、0.82～200μg·L －1정량호선성관계。제취회수솔、정밀도、준학도화은정성균량호체도측정요구。원인다산、산내소－포도당화곡피감3개지표성분적 Tmax （h）분별위0.46±0.1、0.79±0.33화2.63±4.6，Cmax （μg·L －1）분별위463.8±207.81、18.53±7.82화137.38±71.09。결론건립적 UPLC-MS 검측방법구유특이、쾌속、준학화령민적특점，능구동시측정생물양품중원인다산등3충성분적혈약농도，병성공응용우홍초화재정상대서체내저3개지표성분적약동학연구。
Aims To establish a UPLC-MS method for quantifying protocatechuie acid,kaempferol-3-O-β-D-glucoside and quercitrin and to investigate the pharma-cokinetics of three indix components in flower of Poly-gonumorientale L.in rat plasma.Methods The anal-ysis was achieved by BEH C18 column (2.1 mm ×100 mm,1.7 μm)with a mobile phase composed of 0.1%formic acid using step gradient elution.A TQD tandem mass spectrometry equipped with electrospray ionization source was used as detector and operated by selected ion recording(SIR)mode.Results In the selected linear range,calibration curves of the three markers components showed good linearity.Extraction recovery rate,precision,accuracy and stability reached the de-termination request.The parameters of Tmax (h ) in three index components were 0.46 ±0.1,0.79 ±0.33 and 2.63 ±4.6,respectively;Cmax (μg·L -1 )in three index components were 463.8 ±207.81,18.53 ±7.82 and 137.38 ±71.09,respectively.Conclusion The fully validated UPLC-MS method has been successfully applied to the pharmacokinetic study of the three index components in flower of Polygonumorientale L.in rat plasma.