CAJ | 학술논문

通过模拟长江滩地季节性淹水,以夹竹桃为研究对象,分析其内部生理生化指标对淹水胁迫的响应.结果表明：在淹水胁迫下超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性和丙二醛(MDA)含量明显增加,与对照组相比均有极显著差异(p ＜0.01).随着淹水深度加深,SOD、POD、CAT 活性和 MDA 含量总体上呈现增加趋势,轻度淹水和深度淹水处理间 SOD 活性有显著差异(p ＜0.05),POD 活性有极显著差异(p ＜0.01),而 CAT 活性和 MDA 含量无显著差异.随着淹水时间延长,SOD 活性整体上升,差异增大；POD 活性36 d 时达到最大值,与其它时间段有差异；CAT 活性在轻度淹水处理下随着淹水时间延长差异增大；MDA 含量12 d 时最大,与其它时间段存在极显著差异.淹水胁迫解除后,SOD、POD、CAT 等酶活性和 MDA 含量逐渐降到正常水平.夹竹桃在受到淹水胁迫后,体内氧化自由基的积累能迅速诱导保护酶活性的升高,有效降低毒性物质 MDA 的含量,说明夹竹桃具有极强的耐水淹能力.
통과모의장강탄지계절성엄수,이협죽도위연구대상,분석기내부생리생화지표대엄수협박적향응.결과표명：재엄수협박하초양화물기화매(SOD)、과양화물매(POD)、과양화경매(CAT)활성화병이철(MDA)함량명현증가,여대조조상비균유겁현저차이(p ＜0.01).수착엄수심도가심,SOD、POD、CAT 활성화 MDA 함량총체상정현증가추세,경도엄수화심도엄수처리간 SOD 활성유현저차이(p ＜0.05),POD 활성유겁현저차이(p ＜0.01),이 CAT 활성화 MDA 함량무현저차이.수착엄수시간연장,SOD 활성정체상승,차이증대；POD 활성36 d 시체도최대치,여기타시간단유차이；CAT 활성재경도엄수처리하수착엄수시간연장차이증대；MDA 함량12 d 시최대,여기타시간단존재겁현저차이.엄수협박해제후,SOD、POD、CAT 등매활성화 MDA 함량축점강도정상수평.협죽도재수도엄수협박후,체내양화자유기적적루능신속유도보호매활성적승고,유효강저독성물질 MDA 적함량,설명협죽도구유겁강적내수엄능력.
The physiological and biochemical characteristics of Nerium indicum in different flooding stress were studied by simulating the Yangtze River seasonal flooding.The results showed that superoxide dismutase (SOD)activity,peroxi-dase (POD)activity,catalase (CAT)activity,malondialdehyde (MDA)contents of N .indicum in flooding stress in-creased significantly and there were extremely significant differences (p < 0.01 )compared with the control.With the depth increasing,the activity of SOD,POD,CAT and MDA contents showed increasing trend on the whole.Between the mild flooding and deeper,SOD activity had significant difference (p < 0.05 ),POD activity had extremely significant difference (p <0.01),CAT activity and MDA contents had no significant difference.As flooding time progressed,the activity of SOD increased overall and the difference was increasing.POD activity reached the maximum at 36 d,and there was different from other time.CAT activity in mild flooding had difference with prolonged flooding.MDA contents were the largest at 12 d,and there were significant difference with other time.After flooded-stress removal,the enzyme activ-ity of SOD,POD,CAT and MDA contents were gradually reduced to normal levels.After subjected to flooding stress, the oxidation of free radicals in N .indicum can rapidly induce protective enzyme activity to increase and effectively re-duce the content of MDA .In a word,Oleander has strong water resistance ability.