CAJ | 학술논문

目的：建立高效液相色谱法测定复方桔梗远志麻黄碱片Ⅰ中甘草苷及甘草酸的含量。方法：色谱柱为Agilent 5 HC-C18，流动相为乙腈-0.05%磷酸溶液（梯度洗脱），流速为1.0m l/m i n，甘草苷的检测波长为276n m、甘草酸的检测波长为250n m，柱温为30℃，进样量为20μl。结果：甘草苷和甘草酸的质量浓度分别在4.8274～43.4468、34.5511～310.9597μg/m l范围内与各自峰面积积分呈良好线性关系（r=0.9999、0.9999）；精密度、稳定性、重复性试验的R S D≤0.9%；甘草苷和甘草酸的平均加样回收率分别为99.6%、98.4%，R S D分别为0.9%、1.1%（n=6）。结论：本方法简便，准确，精密度好，适用于复方桔梗远志麻黄碱片Ⅰ中甘草苷及甘草酸的含量测定。
목적：건립고효액상색보법측정복방길경원지마황감편Ⅰ중감초감급감초산적함량。방법：색보주위Agilent 5 HC-C18，류동상위을정-0.05%린산용액（제도세탈），류속위1.0m l/m i n，감초감적검측파장위276n m、감초산적검측파장위250n m，주온위30℃，진양량위20μl。결과：감초감화감초산적질량농도분별재4.8274～43.4468、34.5511～310.9597μg/m l범위내여각자봉면적적분정량호선성관계（r=0.9999、0.9999）；정밀도、은정성、중복성시험적R S D≤0.9%；감초감화감초산적평균가양회수솔분별위99.6%、98.4%，R S D분별위0.9%、1.1%（n=6）。결론：본방법간편，준학，정밀도호，괄용우복방길경원지마황감편Ⅰ중감초감급감초산적함량측정。
Objective To establish a method for the content determination of liquiritin and glycyrrhizic acid in Compound radix platycodi polygala ephedrine tabletsⅠby HPLC. Methods The determination was performed on Agilent 5 HC-C18 column with mobile phase consisted of acetonitrile-0.05%phosphoric acid (gradient elution) at the flow rate of 1.0ml/min. The detection wavelength was set at 262 nm for liquiritin and 250nm for glycyrrhizic acid. The column temperature was 30℃. The sample size was 20 μl. Results The linear range were 4.8274~43.4468μg/ml of liquiritin (r=0.999 9), 34.5511~310.9597μg/ml for glycyrrhizic acid (r=0.999 9) . RSDs of precision, stabillity and repeatability tests were≤0.9%. The average recoveries were 99.6%(RSD=0.9%, n=6) and 98.4%(RSD=1.1%, n=6). Conclusions This method is simple and accurate with good precision .It can be used for the contents determination of liquiritin and glycyrrhizic acid in Compound radix platycodi polygala ephedrine tabletsⅠ.