CAJ | 학술논문

测定骨刺片中柚皮苷、芍药苷的含量。采用HPLC法测定柚皮苷、芍药苷的含量。色谱柱： Inertsil ODS－SP C18（250 mm×4.60 mm，5μm）；检测波长：283 nm；流动相：乙腈－0.1％醋酸溶液梯度洗脱；柱温30℃，流速0.8 mL／min；进样量：10μL· mL－1。柚皮苷：线性范围为35.19μg· mL－1～1.7595 mg· mL－1， r＝0.99990，平均回收率为102.50％， RSD为0.5％（n＝6）。芍药苷：线性范围为35.74μg· mL－1～1.7870 mg· mL－1， r＝0.99990，平均回收率为99.79％， RSD为0.9％（n＝6）。该方法简便、快速、准确，为提高和完善该制剂的质量标准提供了依据。
측정골자편중유피감、작약감적함량。채용HPLC법측정유피감、작약감적함량。색보주： Inertsil ODS－SP C18（250 mm×4.60 mm，5μm）；검측파장：283 nm；류동상：을정－0.1％작산용액제도세탈；주온30℃，류속0.8 mL／min；진양량：10μL· mL－1。유피감：선성범위위35.19μg· mL－1～1.7595 mg· mL－1， r＝0.99990，평균회수솔위102.50％， RSD위0.5％（n＝6）。작약감：선성범위위35.74μg· mL－1～1.7870 mg· mL－1， r＝0.99990，평균회수솔위99.79％， RSD위0.9％（n＝6）。해방법간편、쾌속、준학，위제고화완선해제제적질량표준제공료의거。
To determine the content of Naringin and Paeoniflorin in Gu CI Tablet , Naringin and Paeoniflorin were determined on a Inertsil ODS-SP C18 (250 mm ×4.60 mm, 5 μm) column used with the mobile phase of acetonitrile-0.1%acetic acid solution with gradient elution , 283 nm as the detection wavelength , 30 ℃ as the column temperature and 0.8 mL· min-1 as the flow rate.Result showed that the linear range of Naringin was 35.35 μg· mL-1 ~1.7675 mg· mL-1 ( r=0.99990 ).The average recovery was 102.50%( RSD=0.5%, n=6 ).The linear range of Paeoniflorin was 36.19 μg· mL-1 ~1.8095 mg· mL-1 ( r=0.99990 ).The average recovery was 99.79%( RSD=0.9%, n=6 ).The method is simple , accurate and repeatable , and can provide the basis for the improvement and optimization of the quality standard of Gu CI Tablet.