安徽大学学报(自然科学版)
安徽大學學報(自然科學版)
안휘대학학보(자연과학판)
JOURNAL OF ANHUI UNIVERSITY(NATURAL SCIENCES EDITION)
2015年
5期
96-102
,共7页
邸仕忠%孙小红%罗永统%陈维竟%彭福英%彭福佳%杨飞
邸仕忠%孫小紅%囉永統%陳維竟%彭福英%彭福佳%楊飛
저사충%손소홍%라영통%진유경%팽복영%팽복가%양비
玉米%自交系%SSR标记%指纹图谱
玉米%自交繫%SSR標記%指紋圖譜
옥미%자교계%SSR표기%지문도보
maize%inbred lines%SSR marker%finger printing
选用32对玉米SSR引物检测重庆市部分常用及新选玉米自交系间的遗传差异,基于扩增带型清晰、稳定和易于统计原则,从中筛选10对引物作为构建66个玉米自交系指纹图谱的核心引物.结果表明,共扩增出31个条带,每对引物检测到条带3~4个,平均为3.10个.多态性信息量(PIC值)变幅为0.487~0.723,平均为0.651.相似系数在0.29~0.94之间,平均值为0.62.聚类分析表明,10对引物可将66份玉米自交系区分开来.初步构建了重庆市66个玉米自交系的指纹图谱.
選用32對玉米SSR引物檢測重慶市部分常用及新選玉米自交繫間的遺傳差異,基于擴增帶型清晰、穩定和易于統計原則,從中篩選10對引物作為構建66箇玉米自交繫指紋圖譜的覈心引物.結果錶明,共擴增齣31箇條帶,每對引物檢測到條帶3~4箇,平均為3.10箇.多態性信息量(PIC值)變幅為0.487~0.723,平均為0.651.相似繫數在0.29~0.94之間,平均值為0.62.聚類分析錶明,10對引物可將66份玉米自交繫區分開來.初步構建瞭重慶市66箇玉米自交繫的指紋圖譜.
선용32대옥미SSR인물검측중경시부분상용급신선옥미자교계간적유전차이,기우확증대형청석、은정화역우통계원칙,종중사선10대인물작위구건66개옥미자교계지문도보적핵심인물.결과표명,공확증출31개조대,매대인물검측도조대3~4개,평균위3.10개.다태성신식량(PIC치)변폭위0.487~0.723,평균위0.651.상사계수재0.29~0.94지간,평균치위0.62.취류분석표명,10대인물가장66빈옥미자교계구분개래.초보구건료중경시66개옥미자교계적지문도보.
Genetic diversity of 66 maize inbred lines in Chongqing City was studied using 32 pairs of SSR primers .Ten pairs of primers generating distinct ,steady polymorphic bands were selected from 32 pairs of primers as a set of core primers . Thirty‐one bands were detected at the ten SSR loci .The alleles per locus ranged from 3 to 4 ,with an average of 3.10 .The polymorphism information content (PIC) of SSR markers varied from 0.487 to 0.723 ,with an average of 0.651 .The genetic similarity coefficients ranged from 0.29 to 0.94 ,with an average of 0.62 .Clustering analysis indicated that the 66 inbreds could be distinguished from each other . The finger printing of the 66 maize inbred lines was established using the 10 pairs of primers .