CAJ | 학술논문

目的：探讨在肾透明细胞癌患者血清中 miRNA-106a 表达水平及其与临床病理特征的关系，为后期深入研究提供参考数据。方法选取5例肾透明细胞癌肿瘤组织及瘤旁正常肾组织，对其进行 miRNA 芯片筛测，选出肾癌组织中异常表达的 miRNA；收集28例肾癌患者术前血清样本（肾癌组），收集26例健康体检者血清样本作为对照组，采用实时荧光定量 PCR 技术检测肾癌患者和正常人血清中 miRNA-106a 的表达水平，分析肾癌患者术前血清 miRNA-106a 表达与临床病理特征的关系。结果 miRNA 芯片检测肾细胞癌肿瘤组织中 miRNA-106a 较肿瘤旁肾脏正常组织明显增加，与对照组比较，肾癌组患者血清 miRNA-106a 显著上调，差异有统计学意义；不同性别、年龄、肿瘤直径、Fruhman 分期患者血清 miRNA-106a 表达量差异均无统计学意义。结论 miRNA-106a在肾癌患者血清中高表达，可能对肾细胞癌的诊断具有一定临床价值。
목적：탐토재신투명세포암환자혈청중 miRNA-106a 표체수평급기여림상병리특정적관계，위후기심입연구제공삼고수거。방법선취5례신투명세포암종류조직급류방정상신조직，대기진행 miRNA 심편사측，선출신암조직중이상표체적 miRNA；수집28례신암환자술전혈청양본（신암조），수집26례건강체검자혈청양본작위대조조，채용실시형광정량 PCR 기술검측신암환자화정상인혈청중 miRNA-106a 적표체수평，분석신암환자술전혈청 miRNA-106a 표체여림상병리특정적관계。결과 miRNA 심편검측신세포암종류조직중 miRNA-106a 교종류방신장정상조직명현증가，여대조조비교，신암조환자혈청 miRNA-106a 현저상조，차이유통계학의의；불동성별、년령、종류직경、Fruhman 분기환자혈청 miRNA-106a 표체량차이균무통계학의의。결론 miRNA-106a재신암환자혈청중고표체，가능대신세포암적진단구유일정림상개치。
Objective To detect plasma miRNA-106a expression in patients with renal cell carcinoma and analyze its correlation to the clinicopathological feature and disease diagnosis.Methods To screen out ab-normal expression miRNA in5 renal clear cell carcinoma tumor tissue and adjacent normal tissues using miRNA screening chip.28 serum samples in patients with RCC was collected before surgery,26 healthy controls were included,the expression level of miRNA-106a were detected by real-time PCR.Results Compared with the health control,the expression level of miRNA-106a in tissues with RCC is increased obviously.The serum miRNA-106a expression were up-regulated inin RCC samples.There were no statis-tical significance in miRNA-106a expression difference with gender,age,dismeter of tumor and Fruhman classification.Conclusion The expression level of miRNA-106a is closely related to RCC,miRNA-106a can be regarded as a potential molecular biomarker in RCC.