CAJ | 학술논문

目的：探讨肿瘤肾虚痰瘀证患者血浆中相关的氨基酸代谢变化及其机制。方法选取健康志愿者50例、肿瘤肾虚痰瘀证患者80例和肿瘤非肾虚痰瘀证患者78例。采用高效液相色谱法（High-Performance Liquid Chromatography，HPLC）检测血浆中17种氨基酸含量，分析各组不同氨基酸含量的差异性。结果健康志愿者与肿瘤肾虚痰瘀证患者血浆中将胱氨酸含量没有差异以外，肿瘤肾虚痰瘀证和肿瘤非肾虚痰瘀证患者血浆中其他所有氨基酸含量均明显降低，与健康志愿者比较差异有统计学意义，（P ＜0．05）。肿瘤肾虚痰瘀证患者血浆中天冬氨酸、谷氨酸、丝氨酸、胱氨酸、苯丙氨酸、缬氨酸、蛋氨酸、赖氨酸、异亮氨酸、亮氨酸和酪氨酸11种氨基酸含量均高于肿瘤非肾虚痰瘀证患者，差异有统计学意义，（P ＜0．05）。结论肿瘤肾虚痰瘀证和非肾虚痰瘀证患者血浆中氨基酸代谢的变化过程基本一致，但是这种变化的程度有所不同。采用 HPLC 法进行血浆氨基酸分析可以发现潜在的、与肾虚痰瘀证相关的代谢机制差异。
목적：탐토종류신허담어증환자혈장중상관적안기산대사변화급기궤제。방법선취건강지원자50례、종류신허담어증환자80례화종류비신허담어증환자78례。채용고효액상색보법（High-Performance Liquid Chromatography，HPLC）검측혈장중17충안기산함량，분석각조불동안기산함량적차이성。결과건강지원자여종류신허담어증환자혈장중장광안산함량몰유차이이외，종류신허담어증화종류비신허담어증환자혈장중기타소유안기산함량균명현강저，여건강지원자비교차이유통계학의의，（P ＜0．05）。종류신허담어증환자혈장중천동안산、곡안산、사안산、광안산、분병안산、힐안산、단안산、뢰안산、이량안산、량안산화락안산11충안기산함량균고우종류비신허담어증환자，차이유통계학의의，（P ＜0．05）。결론종류신허담어증화비신허담어증환자혈장중안기산대사적변화과정기본일치，단시저충변화적정도유소불동。채용 HPLC 법진행혈장안기산분석가이발현잠재적、여신허담어증상관적대사궤제차이。
Objective To investigate metabolic signatures in plasma of cancer patients with ShenXu-TanYu syndrome using plasma-free amino acid profiles,and identify the underlying mechanism of ShenXu-TanYu syndrome in traditional Chinese medicine.Methods Case group were selected from 80 cancer patients with ShenXu-TanYu or 78 non ShenXu-TanYu)and 50 cases from healthy volunteers were selected as control group,plasma samples were analyzed using high-performance liquid chromatography (HPLC).Spectral profiles were subjected to Student′s t-tests to assess statistical significance.Results Compared with the healthy group,the levels of amino acids decreased significantly in plasma of patients with ShenXu-TanYu and non ShenXu-TanYu syndrome except cystine in patients with ShenXu-TanYu (all P <0.05 except cys-tine).Compared with non ShenXu-TanYu syndrome,patients with ShenXu-TanYu showed high concen-trations of aspartic acid,glutamic acid,serine,cysteine,phenylalanine,valine,methionine,lysine,iso-leucine,leucine and tyrosine (all P < 0.05).The remaining plasma amino acids showed no significant differences (all P >0.05).Conclusion Plasma-free amino acid profiling could potentially assist in under-standing and determining ShenXu-TanYu in traditional Chinese medicine.A HPLC-based metabonomic platform could be a powerful tool for the classification of different symptoms in traditional medicine.