CAJ | 학술논문

目的：探讨咖啡酸苯乙酯（CAPE）对人结肠癌 HT-29细胞 FAK、ERK 及 Caspase-3表达的影响。方法采用不同浓度（2.5、5.0、7.5、10μg·ml-1）的 CAPE 处理体外培养的结肠癌 HT-29细胞后，应用 RT-PCR法检测 FAK、ERK 和 Caspase-3 mRNA 表达水平，采用免疫组织化学方法检测 FAK 和 ERK 的蛋白表达变化。结果不同浓度的 CAPE 作用于 HT-29细胞24 h 后，其 FAK 和 ERK 的 mRNA 表达随 CAPE 浓度增加而下降，Caspase-3的 mRNA 表达随 CAPE 浓度增加而上升。人结肠癌 HT-29细胞中 FAK 和 ERK 蛋白阳性表达定位于细胞浆，经10μg·mL-1的 CAPE 作用24 h 后，FAK 和 ERK 的蛋白表达减少。结论CAPE 可下调HT-29细胞 FAK、ERK 的 mRNA 和蛋白表达，上调 Caspase-3的 mRNA 表达。
목적：탐토가배산분을지（CAPE）대인결장암 HT-29세포 FAK、ERK 급 Caspase-3표체적영향。방법채용불동농도（2.5、5.0、7.5、10μg·ml-1）적 CAPE 처리체외배양적결장암 HT-29세포후，응용 RT-PCR법검측 FAK、ERK 화 Caspase-3 mRNA 표체수평，채용면역조직화학방법검측 FAK 화 ERK 적단백표체변화。결과불동농도적 CAPE 작용우 HT-29세포24 h 후，기 FAK 화 ERK 적 mRNA 표체수 CAPE 농도증가이하강，Caspase-3적 mRNA 표체수 CAPE 농도증가이상승。인결장암 HT-29세포중 FAK 화 ERK 단백양성표체정위우세포장，경10μg·mL-1적 CAPE 작용24 h 후，FAK 화 ERK 적단백표체감소。결론CAPE 가하조HT-29세포 FAK、ERK 적 mRNA 화단백표체，상조 Caspase-3적 mRNA 표체。
Objective To explore the effects of caffeic acid phenethyl ester (CAPE) on the expressions of caspase-3, FAK and ERK mRNA in colorectal cancer cell line HT-29. Methods The colorectal cancer cell line HT-29 was treated with CAPE at serial concentrations (2.5, 5.0, 7.5, 10 μg·ml-1). The expressions of FAK, ERK and Caspase-3 mRNA were determined by RT-PCR. Immunohistochemistry was used to evaluate the protein levels of FAK and ERK in HT-29 cells treated by CAPE. Results After the HT-29 cells were treated with different concentrations (2.5, 5.0, 7.5, 10 μg·ml-1) of CAPE for 24 h, the mRNA expressions of FAK and ERK in HT-29 cells were down-regulated in a concentration-depend-ent manner with the increase of CAPE concentrations from 2.5 to 10 μg·ml-1, but the Caspase-3 mRNA was up-regulated with the increase of CAPE concentrations. The FAK and ERK protein were localized in cytoplasm of HT-29 cells. The treatment with CAPE (10.0 μg·ml-1) for 24 h was associated with the decreased expression of the FAK and ERK protein. Conclusions CAPE could down-regulate the mRNA and protein expressions of FAK and ERK, but up-regulate the mRNA expression of Caspase-3.