天津医药
天津醫藥
천진의약
TIANJIN MEDICAL JOURNAL
2015年
9期
996-999,1000
,共5页
刘佳宇%赵妍蕊%张丽娜%闫晔%郑红
劉佳宇%趙妍蕊%張麗娜%閆曄%鄭紅
류가우%조연예%장려나%염엽%정홍
微RNAs%卵巢肿瘤%上皮-间质转化%上皮源性%miR-200a%miR-141%miR-205%miR-34a
微RNAs%卵巢腫瘤%上皮-間質轉化%上皮源性%miR-200a%miR-141%miR-205%miR-34a
미RNAs%란소종류%상피-간질전화%상피원성%miR-200a%miR-141%miR-205%miR-34a
microRNAs%ovarian neoplasms%epithelial-mesenchymal transition%epithelial%miR-200a%miR-141%miR-205%miR-34a
目的：分析上皮源性卵巢癌（EOC）组织中miR-200a、miR-141、miR-205和miR-34a的表达及其临床意义。方法44例EOC患者按FIGO分期分为FIGOⅠ~Ⅱ（FIGO早期组）组15例和FIGOⅢ~Ⅳ（FIGO晚期组）组29例，用实时荧光定量PCR定量比较2组4种miRNAs的表达差异并考察4种miRNAs表达间的关联。以各组miRNAs表达的中位数将EOC患者分为miRNA高表达组和低表达组，比较2组患者间的生存差异。按年龄、FIGO分期、术后是否有残余瘤及术后化疗情况分组，进行Kaplan-Meier生存分析和Cox多因素分析。结果 FIGO晚期组miR-141的表达高于早期组（P=0.036）。miR-141的表达与miR-200a、miR-205呈正相关，与miR-34a呈负相关（均P<0.05）；miR-200a与miR-205的表达呈正相关（P<0.05）。生存分析显示，miR-200a的表达低，卵巢癌患者无进展生存期短（P=0.035），FIGO早期组生存率高于FIGO晚期组（P<0.05）。miR-200a的表达水平、FIGO分期以及年龄与卵巢癌患者总生存期和无进展生存期有关，miR-141、miR-205和miR-34a的表达水平、术后残余瘤以及化疗与患者生存无关。结论 miR-200a的表达与卵巢癌患者预后有关，可能是卵巢癌患者预后预测的独立影响指标。
目的：分析上皮源性卵巢癌（EOC）組織中miR-200a、miR-141、miR-205和miR-34a的錶達及其臨床意義。方法44例EOC患者按FIGO分期分為FIGOⅠ~Ⅱ（FIGO早期組）組15例和FIGOⅢ~Ⅳ（FIGO晚期組）組29例，用實時熒光定量PCR定量比較2組4種miRNAs的錶達差異併攷察4種miRNAs錶達間的關聯。以各組miRNAs錶達的中位數將EOC患者分為miRNA高錶達組和低錶達組，比較2組患者間的生存差異。按年齡、FIGO分期、術後是否有殘餘瘤及術後化療情況分組，進行Kaplan-Meier生存分析和Cox多因素分析。結果 FIGO晚期組miR-141的錶達高于早期組（P=0.036）。miR-141的錶達與miR-200a、miR-205呈正相關，與miR-34a呈負相關（均P<0.05）；miR-200a與miR-205的錶達呈正相關（P<0.05）。生存分析顯示，miR-200a的錶達低，卵巢癌患者無進展生存期短（P=0.035），FIGO早期組生存率高于FIGO晚期組（P<0.05）。miR-200a的錶達水平、FIGO分期以及年齡與卵巢癌患者總生存期和無進展生存期有關，miR-141、miR-205和miR-34a的錶達水平、術後殘餘瘤以及化療與患者生存無關。結論 miR-200a的錶達與卵巢癌患者預後有關，可能是卵巢癌患者預後預測的獨立影響指標。
목적：분석상피원성란소암（EOC）조직중miR-200a、miR-141、miR-205화miR-34a적표체급기림상의의。방법44례EOC환자안FIGO분기분위FIGOⅠ~Ⅱ（FIGO조기조）조15례화FIGOⅢ~Ⅳ（FIGO만기조）조29례，용실시형광정량PCR정량비교2조4충miRNAs적표체차이병고찰4충miRNAs표체간적관련。이각조miRNAs표체적중위수장EOC환자분위miRNA고표체조화저표체조，비교2조환자간적생존차이。안년령、FIGO분기、술후시부유잔여류급술후화료정황분조，진행Kaplan-Meier생존분석화Cox다인소분석。결과 FIGO만기조miR-141적표체고우조기조（P=0.036）。miR-141적표체여miR-200a、miR-205정정상관，여miR-34a정부상관（균P<0.05）；miR-200a여miR-205적표체정정상관（P<0.05）。생존분석현시，miR-200a적표체저，란소암환자무진전생존기단（P=0.035），FIGO조기조생존솔고우FIGO만기조（P<0.05）。miR-200a적표체수평、FIGO분기이급년령여란소암환자총생존기화무진전생존기유관，miR-141、miR-205화miR-34a적표체수평、술후잔여류이급화료여환자생존무관。결론 miR-200a적표체여란소암환자예후유관，가능시란소암환자예후예측적독립영향지표。
Objective To determine the expressions of miR-200a, miR-141, miR-205 and miR-34a in epithelial ovarian cancer (EOC) samples and to explore their clinical significance. Methods According to FIGO staging, 44 EOC pa?tients were divided into two groups:early FIGO stage (stageⅠ-Ⅱ, n=15) and late FIGO stage (stageⅢ-Ⅳ, n=29). Expres?sions of 4 miRNAs were detected by real time quantitative PCR, and were compared between two groups. The correlation of 4 miRNAs was calculated. EOC patients were divided into high miRNA expression group and low expression group according to the median value of miRNAs expression. Kaplan-Meier survival analysis and Cox multivariate analysis were used to com?pare the age, FIGO state, tumor residual after operation and post-operative chemotherapy of ovarian cancer between two groups. Results The expression of miR-141 was elevated in stagesⅢandⅣcompared with that of stagesⅠand Ⅱ(P=0.036). There was a positive correlation between expression of miR-141, miR-200a and miR-205, but a negative correlation with miR-34a (P<0.05). There was a positive correlation between miR-200a and miR-205 (P<0.05). Lower miR-200a ex?pression was associated with shorter progress free survival in ovarian cancer analyzed by log-rank test ( P=0.035). The sur?vival rate was significantly higher in FIGO stages ⅠandⅡthan that of FIGO stagesⅢandⅣ(P<0.05). Cox regression analysis revealed that miR-200a, FIGO stage and age were influential factors of overall survival time and progress-free sur?vival time of ovarian cancer, while miR-141, miR-205, miR-34a and tumor residual after operation and post-operative che?motherapy were not influential factors. Conclusion The expression of miR-200a is closely correlated with the progress and prognosis of ovarian cancer and may be used as an independent indicator for ovarian cancer prognosis.
