CAJ | 학술논문

目的：观察缺氧／复氧对脂多糖诱导人牙周膜成纤维细胞表达环氧合酶－2、前列腺素E 2的影响。方法用1μg ／mL 脂多糖诱导的人牙周膜成纤维细胞，在1％缺氧浓度下培养6、12、24、48 h，同时分别在缺氧刺激后复氧24 h，用Western Blotting 和ELISA 检测环氧合酶－2和前列腺素E 2的表达。结果缺氧组环氧合酶－2的表达高于对照组，并在24 h 达到高峰，复氧组环氧合酶－2的表达较缺氧组和对照组均增加；前列腺素E 2的表达在复氧组高于对照组与缺氧组，但在缺氧组和对照组之间无统计学差异。结论缺氧能影响脂多糖诱导人牙周膜成纤维细胞环氧合酶－2的表达，复氧能增强环氧合酶－2和前列腺素E 2的表达。缺氧／复氧对环氧合酶－2和前列腺素E 2表达的影响在牙周炎的发展中有重要影响。
목적：관찰결양／복양대지다당유도인아주막성섬유세포표체배양합매－2、전렬선소E 2적영향。방법용1μg ／mL 지다당유도적인아주막성섬유세포，재1％결양농도하배양6、12、24、48 h，동시분별재결양자격후복양24 h，용Western Blotting 화ELISA 검측배양합매－2화전렬선소E 2적표체。결과결양조배양합매－2적표체고우대조조，병재24 h 체도고봉，복양조배양합매－2적표체교결양조화대조조균증가；전렬선소E 2적표체재복양조고우대조조여결양조，단재결양조화대조조지간무통계학차이。결론결양능영향지다당유도인아주막성섬유세포배양합매－2적표체，복양능증강배양합매－2화전렬선소E 2적표체。결양／복양대배양합매－2화전렬선소E 2표체적영향재아주염적발전중유중요영향。
Objective To study the effect of hypoxia / reoxygenation on the the expressions of COX-2 and PGE 2 in lipopolysaccha-ride-inducedind human periodontal ligament cells. Methods Human periodontal ligament cells were first induced by 1 μg / mL lipopo-lysaccharide. Then cells were divided into two experiment groups:hypoxia group and reoxygenation group. In the hypoxia group,cells were cultured in 1% O2 for 6,12,24,48 h,respectively. In the re-oxygenation group,cells were first cultured under the samecondi-tion as the hypoxia group and then returned to normoxic condition for 24 hours. The expression of COX-2 was detected by Wesrtern Blootting and PGE2 was observed by ELISA. Results The expression of COX-2 was higher in the hypoxia group compared to the con-trol group and reached the peak at 24 h. Besides,the expression of COX-2 significantly increased in the reoxygenation group compared to the hypoxia group and the control group. The expression of PGE2 was higher than that in the hypoxia group and the control group. But there was no significant difference between the hypoxia group and the control group. Conclusions Hypoxia could influence the ex-pression of COX-2 in lipopolysaccharide-Induced human periodontal ligament cells but does not affect the expression of PGE2 . Reoxy-genation could increase the expressions of COX-2 and PGE2 . The hypoxia / reoxygenation condition might play an important role in peri-odontitis.