CAJ | 학술논문

目的：探讨干细胞移植对缺血性二尖瓣关闭不全模型犬心肌组织中平滑肌肌动蛋白（smooth muscle actin，SMA）、碱性成纤维细胞生长因子（basic fibroblast growth factor，bFGF）、血管内皮生长因子（vascular endothelial growth factor，VEGF）表达的影响。方法24只健康的雄性实验犬，随机选取6只为正常组，余18只建立二尖瓣关闭不全模型，模型成功后分为模型组5只、实验组6只和对照组6只。实验组向缺血心肌灌注2 mL干细胞悬液，对照组灌注等量不含干细胞的DMEM培养基。检测各组犬的心功能、心肌组织SMA、bFGF、VEGF表达水平并进行分析。结果与对照组及模型组相比较，实验组心功能改善更为明显，表现为心输出量（cardiac output，CO）以及每搏输出量（stroke volume，SV）显著升高（P＜0．05）；心肌组织α－SMA含量明显降低（P＜0．05）；心肌组织bFGF含量明显升高（P＜0．05）；心肌组织VEGF含量明显升高（P＜0．05）。结论干细胞移植能够抑制缺血性二尖瓣关闭不全模型犬心肌组织中SMA表达，促进bFGF和VEGF表达，对心功能有改善作用。
목적：탐토간세포이식대결혈성이첨판관폐불전모형견심기조직중평활기기동단백（smooth muscle actin，SMA）、감성성섬유세포생장인자（basic fibroblast growth factor，bFGF）、혈관내피생장인자（vascular endothelial growth factor，VEGF）표체적영향。방법24지건강적웅성실험견，수궤선취6지위정상조，여18지건립이첨판관폐불전모형，모형성공후분위모형조5지、실험조6지화대조조6지。실험조향결혈심기관주2 mL간세포현액，대조조관주등량불함간세포적DMEM배양기。검측각조견적심공능、심기조직SMA、bFGF、VEGF표체수평병진행분석。결과여대조조급모형조상비교，실험조심공능개선경위명현，표현위심수출량（cardiac output，CO）이급매박수출량（stroke volume，SV）현저승고（P＜0．05）；심기조직α－SMA함량명현강저（P＜0．05）；심기조직bFGF함량명현승고（P＜0．05）；심기조직VEGF함량명현승고（P＜0．05）。결론간세포이식능구억제결혈성이첨판관폐불전모형견심기조직중SMA표체，촉진bFGF화VEGF표체，대심공능유개선작용。
Objective To analyze effect of stem cell transplantation on the expression of smooth muscle actin (SMA), basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF) in the myocardium of dogs with ischemic mitral regurgitation.Methods 24 healthy male experiment dogs were selected, 6 were collected randondy as normal group, the others were used to construct mitral regurgitation model.After that, the modelized dogs were divided into three groups, 5 dogs in model group, 6 dogs in experiment group and 6 in control group.The experimental group were given 2 mL stem cells suspension to ischemic myocardial, control group was infused without stem cells in DMEM medium, and then the SMA, bFGF and VEGF expression levels of myocardial tissues in each groups were detected and analyzed.ResuIts Compared with control group and model group, the heart function of experiment group were improved more obviously, such as cardiac output (CO) and stroke volume (SV) increased significantly (P<0.05).Myocardialα-SMA were significantly lower (P<0.05).bFGF contents in myocardial tissue increased significantly (P<0.05).The content of VEGF in myocardial tissue significantly increased (P<0.05).ConcIusions Stem cell transplantation can inhibit the expression of SMA in myocardium of ischemic mitral regurgitation, and promote the expression of bFGF and VEGF, has a better effect on heart function.