泸州医学院学报
瀘州醫學院學報
로주의학원학보
JOURNAL OF LUZHOU MEDICAL COLLEGE
2015年
4期
341-343
,共3页
小动物PET/CT%麻黄碱%棕色脂肪%标准化摄取值
小動物PET/CT%痳黃堿%棕色脂肪%標準化攝取值
소동물PET/CT%마황감%종색지방%표준화섭취치
Small-animal PET/CT%Ephedrine(Eph)%Brown adipose tissue%Standardised uptake value
目的：：采用小动物PET/CT研究不同剂量麻黄碱(Ephedrine，Eph)与温度对棕色脂肪(Brown Adipose Tissue，BAT)摄取18F-氟代脱氧葡萄糖(18F-fluoro-deoxyglucose，18F-FDG)的影响。方法：将昆明小鼠腹腔注射18F-FDG或18F-FDG+Eph(40 mg/kg、80 mg/kg 和160 mg/kg)，放置于不同实验温度条件下，1 h后采用小动物 PET/CT 对小鼠进行全身PET/CT扫描，通过Inveon Research Workplace(IRW)软件对小鼠肩胛处BAT摄取18F-FDG的标准化摄取值(Standardised uptake value，SUV)进行分析。结果：25℃和15℃未使用Eph干预的BAT SUV值分别为1.67±0.49和2.7±0.26；25℃和15℃使用Eph(40 mg/kg)干预BAT SUV值分别为2.20±0.36和5.97±0.21。温度为25℃，Eph分别为40 mg/kg、80 mg/kg和160 mg/kg时BAT SUV值分别为2.20±0.36、3.33±0.31和6.73±0.50。结论：低温可以刺激BAT的能量代谢增高，同时随着Eph的剂量增高BAT的能量代谢程度也随之增加。小动物PET/CT能有效检测小鼠体内肩胛处BAT对18F-FDG的代谢情况。
目的：：採用小動物PET/CT研究不同劑量痳黃堿(Ephedrine，Eph)與溫度對棕色脂肪(Brown Adipose Tissue，BAT)攝取18F-氟代脫氧葡萄糖(18F-fluoro-deoxyglucose，18F-FDG)的影響。方法：將昆明小鼠腹腔註射18F-FDG或18F-FDG+Eph(40 mg/kg、80 mg/kg 和160 mg/kg)，放置于不同實驗溫度條件下，1 h後採用小動物 PET/CT 對小鼠進行全身PET/CT掃描，通過Inveon Research Workplace(IRW)軟件對小鼠肩胛處BAT攝取18F-FDG的標準化攝取值(Standardised uptake value，SUV)進行分析。結果：25℃和15℃未使用Eph榦預的BAT SUV值分彆為1.67±0.49和2.7±0.26；25℃和15℃使用Eph(40 mg/kg)榦預BAT SUV值分彆為2.20±0.36和5.97±0.21。溫度為25℃，Eph分彆為40 mg/kg、80 mg/kg和160 mg/kg時BAT SUV值分彆為2.20±0.36、3.33±0.31和6.73±0.50。結論：低溫可以刺激BAT的能量代謝增高，同時隨著Eph的劑量增高BAT的能量代謝程度也隨之增加。小動物PET/CT能有效檢測小鼠體內肩胛處BAT對18F-FDG的代謝情況。
목적：：채용소동물PET/CT연구불동제량마황감(Ephedrine，Eph)여온도대종색지방(Brown Adipose Tissue，BAT)섭취18F-불대탈양포도당(18F-fluoro-deoxyglucose，18F-FDG)적영향。방법：장곤명소서복강주사18F-FDG혹18F-FDG+Eph(40 mg/kg、80 mg/kg 화160 mg/kg)，방치우불동실험온도조건하，1 h후채용소동물 PET/CT 대소서진행전신PET/CT소묘，통과Inveon Research Workplace(IRW)연건대소서견갑처BAT섭취18F-FDG적표준화섭취치(Standardised uptake value，SUV)진행분석。결과：25℃화15℃미사용Eph간예적BAT SUV치분별위1.67±0.49화2.7±0.26；25℃화15℃사용Eph(40 mg/kg)간예BAT SUV치분별위2.20±0.36화5.97±0.21。온도위25℃，Eph분별위40 mg/kg、80 mg/kg화160 mg/kg시BAT SUV치분별위2.20±0.36、3.33±0.31화6.73±0.50。결론：저온가이자격BAT적능량대사증고，동시수착Eph적제량증고BAT적능량대사정도야수지증가。소동물PET/CT능유효검측소서체내견갑처BAT대18F-FDG적대사정황。
Objective To investigato the effect of different doses of Ephedrine(Eph) and temperature on 18F-FDG uptake in brown adipose tissue (BAT) of Kungming mice using small-animal PET/CT. Methods: 18F-FDG or a mixture of 18F-FDG and Eph (40 mg/kg, 80 mg/kg or 160 mg/kg of body weight) were injected into the peritoneal cavity of mice at different temperatures. One hour after injection, the BAT activity was assessed by measuring SUV with Micro PET/CT. ResultsIn mice without ingection of Eph, the SUV of 18F-FDG, was 1.67 ±0.49 at 25℃, and 2.7±0.26 at 15℃, respectively. In mice injected with 40 mg/kg of Eph, the SUV was 2.20 ±0.36 at 25 ℃, and 5.97 ± 0.21 at 15 ℃, respectively. At 25 ℃, mice injected with 40 mg/kg, 80 mg/kg and 160 mg/kg of Eph, the The SUV of 18F-FDG was 2.20± 0.36, 3.33 ±0.31 and 6.73±0.50 respectively. ConclusionThe cold exposure could cause a greater increase in 18F-FDG uptake in BAT, and the metabolism level of BAT was will increase with the increased dose off. Small-animal PET/C is suitable for the study of 18F-FDG metabolism in interscapular BAT of mice.
