CAJ | 학술논문

目的：研究大鲵皮肤黏液糖蛋白提取纯化工艺以及体外对人肺癌细胞A549活性的影响。方法利用碱提取和DEAE－52离子交换层析与Sephadex G－100凝胶层析分离纯化大鲵黏液糖蛋白；并采用MTT比色法体外检测大鲵黏液糖蛋白对人肺癌细胞A549的抑制率。结果黏液糖蛋白中的总糖含量为4．23％，经SDS－PAGE电泳检测，糖蛋白的分子量在30 kDa左右，为单一纯品。随糖蛋白纯品浓度从1、10、20、40μg／mL逐渐增加，糖蛋白对A549细胞的抑制率逐渐增大；当糖蛋白浓度为40μg／mL，作用24 h时，对A549细胞的抑制率可达85．66％，作用48 h时，可达92．32％。与阳性对照紫杉醇相比，大鲵皮肤黏液糖蛋白对人肺癌细胞A549有显著的抑制作用。结论大鲵黏液糖蛋白对人肺癌细胞具有明显的抑制作用，可为抗肺癌药物的开发提供理论依据。
목적：연구대예피부점액당단백제취순화공예이급체외대인폐암세포A549활성적영향。방법이용감제취화DEAE－52리자교환층석여Sephadex G－100응효층석분리순화대예점액당단백；병채용MTT비색법체외검측대예점액당단백대인폐암세포A549적억제솔。결과점액당단백중적총당함량위4．23％，경SDS－PAGE전영검측，당단백적분자량재30 kDa좌우，위단일순품。수당단백순품농도종1、10、20、40μg／mL축점증가，당단백대A549세포적억제솔축점증대；당당단백농도위40μg／mL，작용24 h시，대A549세포적억제솔가체85．66％，작용48 h시，가체92．32％。여양성대조자삼순상비，대예피부점액당단백대인폐암세포A549유현저적억제작용。결론대예점액당단백대인폐암세포구유명현적억제작용，가위항폐암약물적개발제공이론의거。
Objective To study the appearance of skin mucous glycoprotein in vitro on the activity of human lung cancer cells A549. Methods Used alkali extraction and DEAE-52 ion exchange chromatography and Sephadex G-100 gel chromatography purification salamander mucous glycoprotein; Salamander mucous glycoprotein inhibition of human lung cancer cells A549 was detected by MTT colorimetric method in vitro.ResuIts It showed that the total sugar content in the appearance of mucus was 4.23%, the relatively pure glycoprotein component, by SDS protein electrophoresis tests, it contained a single glycoprotein component, its molecular weight was about 30 kDa.With glycoprotein pure concentration increased from 1,10, 20,40μg/mL, the glycoprotein inhibition rate of A549 cells increased; when the glycoprotein concentration was 40 μg/mL, for 24 h action, the inhibition rate of A549 cells was up to 85.66 %, while the role of 48 h, the inhibition rate of A549 cells was up to 92.32%.Inhibition effect of mucus glycoprotein on A549 cell compared with positive control drug, had significant difference.ConcIusion Salamander mucus glycoproteins of human lung cancer cells has obvious inhibitory effect, can provide theoretical basis for the development of lung cancer drug resistance.