CAJ | 학술논문

目的：探讨鸦葱总黄酮对乙型肝炎病毒的体外抑制作用。方法通过四甲基偶氮唑盐（ MTT）法观察鸦葱总黄酮对HepG2．2．15细胞的影响；采用酶联免疫吸咐（ ELISA ）法检测分析鸦葱总黄酮对HepG2．2．15细胞分泌表面抗原（ HBsAg ）和E抗原（HBeAg）的抑制作用；采用荧光定量 PCR 法检测鸦葱总黄酮对乙型肝炎病毒核酸（ HBV－DNA）的影响。结果鸦葱总黄酮对HepG2．2．15细胞的半数毒性浓度（TC50）为0．603 mg／mL；鸦葱总黄酮高中低3个无毒剂量（0．062、0．125、0．250 mg／mL）均能明显降低HepG2．2．15细胞培养液中HBsAg、HBeAg的含量和HBV－DNA数量，最大抑制率分别为89％、33％及43％。结论鸦葱总黄酮具有显著的抗乙型肝炎病毒作用。
목적：탐토아총총황동대을형간염병독적체외억제작용。방법통과사갑기우담서염（ MTT）법관찰아총총황동대HepG2．2．15세포적영향；채용매련면역흡부（ ELISA ）법검측분석아총총황동대HepG2．2．15세포분비표면항원（ HBsAg ）화E항원（HBeAg）적억제작용；채용형광정량 PCR 법검측아총총황동대을형간염병독핵산（ HBV－DNA）적영향。결과아총총황동대HepG2．2．15세포적반수독성농도（TC50）위0．603 mg／mL；아총총황동고중저3개무독제량（0．062、0．125、0．250 mg／mL）균능명현강저HepG2．2．15세포배양액중HBsAg、HBeAg적함량화HBV－DNA수량，최대억제솔분별위89％、33％급43％。결론아총총황동구유현저적항을형간염병독작용。
Objective To investigate anti-HBV effect of total flavonoids in Scorzonera austriaca wild (TFSA) in vitro.Methods MTT assay was used to observe the effect of TFSA on HepG2.2.15 cells, ELISA assay was used to detect the inhibition on HBsAg and HBeAg secretion from HepG2.2.15 cells and RTFQ-PCR assay was used to detect the inhibition rates of HBV-DNA.ResuIts The TC50 of TFSA on HepG2.2.15 cells was 0.603 mg/mL . TFSA significantly reduced the content of HBsAg and HBeAg and the numbers of HBV-DNA in the HepG2.2.15 cell cultural supernatants under nontoxic concentrations (0.062, 0.125, 0.250 mg/mL), and the maximal inhibitory rate was 89%, 33% and 43%, respectively. ConcIusion TFSA have anti-HBV effects in vitro.