价值工程
價值工程
개치공정
VALUE ENGINEERING
2015年
27期
163-167,168
,共6页
海马%蛋白质%提取分离%SDS-PAGE
海馬%蛋白質%提取分離%SDS-PAGE
해마%단백질%제취분리%SDS-PAGE
hippocampus%protein%extraction and separation%SDS-PAGE
探讨不同处理方式海马蛋白的差异，并对其部分生化特性进行研究。利用硫酸铵分步沉淀、聚丙烯酰胺凝胶电泳法(SDS-PAGE)、bandscan软件、红外图谱对蒸馏水复水，醋酸铵复水，烘烤炮制获得的海马蛋白质进行比较分析。结果发现，烘烤炮制的海马蛋白质结构被破坏，凝胶图谱无条带，而不同复水方法凝胶图谱具有显著差异；通过计算分子量得知海马中蛋白质主要为肌肉蛋白质中的肌浆蛋白与肌原纤维蛋白；对海马蛋白质的红外指纹图谱进行分析，海马不同处理方式提纯后的蛋白质与烘烤炮制的海马全蛋白质的图谱存在区别。
探討不同處理方式海馬蛋白的差異，併對其部分生化特性進行研究。利用硫痠銨分步沉澱、聚丙烯酰胺凝膠電泳法(SDS-PAGE)、bandscan軟件、紅外圖譜對蒸餾水複水，醋痠銨複水，烘烤砲製穫得的海馬蛋白質進行比較分析。結果髮現，烘烤砲製的海馬蛋白質結構被破壞，凝膠圖譜無條帶，而不同複水方法凝膠圖譜具有顯著差異；通過計算分子量得知海馬中蛋白質主要為肌肉蛋白質中的肌漿蛋白與肌原纖維蛋白；對海馬蛋白質的紅外指紋圖譜進行分析，海馬不同處理方式提純後的蛋白質與烘烤砲製的海馬全蛋白質的圖譜存在區彆。
탐토불동처리방식해마단백적차이，병대기부분생화특성진행연구。이용류산안분보침정、취병희선알응효전영법(SDS-PAGE)、bandscan연건、홍외도보대증류수복수，작산안복수，홍고포제획득적해마단백질진행비교분석。결과발현，홍고포제적해마단백질결구피파배，응효도보무조대，이불동복수방법응효도보구유현저차이；통과계산분자량득지해마중단백질주요위기육단백질중적기장단백여기원섬유단백；대해마단백질적홍외지문도보진행분석，해마불동처리방식제순후적단백질여홍고포제적해마전단백질적도보존재구별。
To investigate the difference of hippocampal proteins by difference processing pathways and to study its biochemical properties. Analysis on characteristic from hippocampal protein by difference processing pathways (rehydration by water, rehydration by ammonium acetate, processing by bake) by method of fractional precipitation, SDS-PAGE, bandscan5.0, Infrared spectrometry. It was found that the protein form hippocampus which processing by bake structure was destroyed, there was no gel band pattern. The protein of hippocampal rehydration by water was different from rehydration by ammonium acetate significantly. Calculated molecular weight of the protein in the hippocampus,we can known that the major protein in hippocampus were SPP and MFP. There were some differences between the IR spectra of hippocampus proteins by difference processing pathways.
