CAJ | 학술논문

目的：研究小白菊内酯（parthenolide，PTN）对多柔比星（doxorubicin，DOX）肾损伤大鼠肾功能的保护作用和其氧化应激的机制。方法48只SD大鼠随机分为6组：空白组、模型组、溶媒组、PTN（1．5、3、6 mg／kg）剂量组，每组8只。尾静脉一次性注射5 mg／kg多柔比星制备肾损伤模型，连续PTN给药7d后取血检测大鼠血清尿素氮（ BUN）和肌酐（ Cr）水平，肾组织中一氧化氮（ nitricoxide ，NO）、丙二醛（ malondialdehyde ， MDA ）、一氧化氮合酶（ nitricoxidesynthase ， NOS ）、谷胱甘肽过氧化物酶（ glutathione peroxidase，GSH）和超氧化物歧化酶（superoxide dismutase，SOD）活性，同时取肾组织用HE染色观察肾组织病理改变。结果模型组大鼠肾脏的组织病理学发生明显变化，表现为嗜碱性变，间质炎性增生、炎性浸润；经PTN干预后，PTN 6 mg／kg组中大鼠肾脏组织病理变化减轻，轻度炎性浸润。 PTN 1．5 mg／kg组、PTN 3 mg／kg组、PTN 6 mg／kg组BUN、Cr含量显著低于模型组（ P＜0．05）；PTN 3 mg／kg组、PTN 6 mg／kg组BUN、Cr含量与空白组相比，差异无统计学意义。与模型组比较，3个PTN剂量组SOD、GSH活性显著升高而MDA水平显著下降（ P＜0．05）；PTN 3 mg／kg组、PTN 6 mg／kg组SOD水平与空白组相比差异无统计学意义，PTN 6 mg／kg组GSH活性、MDA水平与空白组相比差异无统计学意义。与模型组相比，3个PTN剂量组NO水平、NOS活性显著升高（ P＜0．05）；PTN 6 mg／kg组NO水平、NOS活性与空白组相比差异无统计学意义。结论小白菊内酯对多柔比星所致大鼠肾脏损伤具有保护作用，可能与提高肾脏抗氧化应激能力有关。
목적：연구소백국내지（parthenolide，PTN）대다유비성（doxorubicin，DOX）신손상대서신공능적보호작용화기양화응격적궤제。방법48지SD대서수궤분위6조：공백조、모형조、용매조、PTN（1．5、3、6 mg／kg）제량조，매조8지。미정맥일차성주사5 mg／kg다유비성제비신손상모형，련속PTN급약7d후취혈검측대서혈청뇨소담（ BUN）화기항（ Cr）수평，신조직중일양화담（ nitricoxide ，NO）、병이철（ malondialdehyde ， MDA ）、일양화담합매（ nitricoxidesynthase ， NOS ）、곡광감태과양화물매（ glutathione peroxidase，GSH）화초양화물기화매（superoxide dismutase，SOD）활성，동시취신조직용HE염색관찰신조직병리개변。결과모형조대서신장적조직병이학발생명현변화，표현위기감성변，간질염성증생、염성침윤；경PTN간예후，PTN 6 mg／kg조중대서신장조직병리변화감경，경도염성침윤。 PTN 1．5 mg／kg조、PTN 3 mg／kg조、PTN 6 mg／kg조BUN、Cr함량현저저우모형조（ P＜0．05）；PTN 3 mg／kg조、PTN 6 mg／kg조BUN、Cr함량여공백조상비，차이무통계학의의。여모형조비교，3개PTN제량조SOD、GSH활성현저승고이MDA수평현저하강（ P＜0．05）；PTN 3 mg／kg조、PTN 6 mg／kg조SOD수평여공백조상비차이무통계학의의，PTN 6 mg／kg조GSH활성、MDA수평여공백조상비차이무통계학의의。여모형조상비，3개PTN제량조NO수평、NOS활성현저승고（ P＜0．05）；PTN 6 mg／kg조NO수평、NOS활성여공백조상비차이무통계학의의。결론소백국내지대다유비성소치대서신장손상구유보호작용，가능여제고신장항양화응격능력유관。
Objective To observe protective effects of parthenolide ( PTN) in rats with doxorubicin-induced nephropathy and its mechanims. Methods 48 male rats were randomly divided into six groups: blank group, model group, vehicle group, and parthenolide-treated group (1.5, 3, 6 mg/kg).The models of doxorubicin-induced nephropathy were established by tail vein injection of 5 mg/kg doxorubicin for one time.After consecutive treatment of PTN for seven days, the BUN and Cr in serum, and nitricoxide (NO), malondialdehyde (MDA), nitricoxidesynthase (NOS), glutathione peroxidase (GSH), superoxide dismutase (SOD) in renal tissues were detected.The pathological changes of renal tissue was observed by HE method. ResuIts There was significant pathological changes of renal tissue in model group, that showed basophilic change, interstitial inflammatory hyperplasia and inflammatory infiltratio, after PTN 6 mg/kg treatment, the above changes significant improved.The serum BUN and Cr levels in PTN 1.5 mg/kg, PTN 3 mg/kg and PTN 6 mg/kg group were lower than those in model group (P<0.05);there were no significant differences of above indexes between PTN 3 mg/kg, PTN 6 mg/kg group and blank gorup.Compared with model group, the SOD and GSH activity were higher and MDA level was lower in three different dosage of PTN groups (P<0.05); there were no significant differences of SOD activity between PTN 3 mg/kg, PTN 6 mg/kg group and blank gorup; there were no significant differences of GSH activity and MDA level between PTN 6 mg/kg group and blank gorup.The NO level and NOS activity in three different dosage of PTN groups were higher (P<0.05);there were no significant differences of NO level and NOS activity between PTN 6 mg/kg group and blank gorup.ConcIusion Parthenolide has protective effects on doxorubicin-induced nephropathy, and its mechanism might be antioxidative effects.