CAJ | 학술논문

目的：：探讨99mTc-DTPA-脱氧葡萄糖(DG)对非小细胞肺癌荷瘤裸鼠化疗疗效的评价。方法：建模成功后的A549肺癌裸鼠分为2组：A组为对照组(NS)；B组为顺铂组(DDP)。裸鼠化疗疗程完成后分别于次日和第7、14、21、28 d注射99mTc-DTPA-DG 3.7 MBq/只，0.5 h后显像，勾画感兴趣区(ROI)，计算肿瘤/健侧对应部位放射性(T/TN)比值，并测量肿瘤体积。第28 d完成显像后处死裸鼠，测量肿瘤/血液及肿瘤/肌肉放射性比值，计算抑瘤率，并剥离瘤组织，测量质量。结果：肿瘤组织吸收99mTc-DTPA-DG较多，肿瘤/血液放射性比值对照组为3.95，高于顺铂组(0.89，t=3.282，P<0.05)。肿瘤/肌肉放射性比值对照组为6.73，高于顺铂组(0.81，t=4.096，P<0.05)。99mTc-DTPA-DG可使肿瘤组织清晰显像，第28 d顺铂组与对照组肿瘤体积分别为388.64mm3、1052.41mm3，差异有统计学意义(t=4.053，P<0.05)。结论：顺铂对非小细胞肺癌化疗后，99mTc-DTPA-DG显像示肿瘤体积缩小，瘤体内放射性分布降低，99mTc-DTPA-DG显像可用于非小细胞肺癌化疗疗效的评估。
목적：：탐토99mTc-DTPA-탈양포도당(DG)대비소세포폐암하류라서화료료효적평개。방법：건모성공후적A549폐암라서분위2조：A조위대조조(NS)；B조위순박조(DDP)。라서화료료정완성후분별우차일화제7、14、21、28 d주사99mTc-DTPA-DG 3.7 MBq/지，0.5 h후현상，구화감흥취구(ROI)，계산종류/건측대응부위방사성(T/TN)비치，병측량종류체적。제28 d완성현상후처사라서，측량종류/혈액급종류/기육방사성비치，계산억류솔，병박리류조직，측량질량。결과：종류조직흡수99mTc-DTPA-DG교다，종류/혈액방사성비치대조조위3.95，고우순박조(0.89，t=3.282，P<0.05)。종류/기육방사성비치대조조위6.73，고우순박조(0.81，t=4.096，P<0.05)。99mTc-DTPA-DG가사종류조직청석현상，제28 d순박조여대조조종류체적분별위388.64mm3、1052.41mm3，차이유통계학의의(t=4.053，P<0.05)。결론：순박대비소세포폐암화료후，99mTc-DTPA-DG현상시종류체적축소，류체내방사성분포강저，99mTc-DTPA-DG현상가용우비소세포폐암화료료효적평고。
Objective To investigate the feasibility of using 99mTc -DTPA -DG imaging to assess chemotherapeutic efficacy on non-small cell lung cancer(NSLC). Methods:Twenty male nude rats inoculated with A549 cell were randomly divided into two groups. The control group was treated with 2 mL/kg of saline. The DDP group was treated with 2 mL/kg of Cisplatin. Scintigraphic studies were conducted 1, 7, 14, 21 and 28 d after the treatment. The radioactivity ratios of tumor to non-tumor (T/NT) was calculated with the region of interest (ROI) technique. After the last imaging the rats were sacrificed. The radioactivity ratios of tumor/ blood and tumor/muscle were measured. Tumor volume and thereby the inhibition rate in both groups were calculated. ResultsThe tumor cells took more 99mTc-DTPA-DG than normal tissues. The control group had higher tumor/ blood ratio (3.95) and tumor/muscle ratio (6.73) than DDP group (0.89, 0.81, t = 3.282, t =4.096,P<0.05). Tumors were clearly visualized on the 99mTc-DTPA-DG images. The tumor volume of DDP group and contyol group at the 28th day was 388.64 mm3 and, 1052.41 mm3 respectively, which is statistical significant (t = 4.717 , P < 0.05). ConclusionThe decrease of tumor volume and radioactivity ratio after the chemotherapy of DDP can be distinctively demonstrated which prores that the 99mTc-DTPA-DG images could be used to evaluate the chemo-therapeutic efficacy on non-small cell lung cancer.