CAJ | 학술논문

目的：探讨肺宁颗粒防治大鼠急性放射性肺损伤（ radiation induced lung injur ，RILI）及对细胞因子转化生长因子β1（transforming growth factor－β1，TGF－β1）、白介素－1（interleukin－1，IL－1）、白介素－6（interleukin－6，IL－6）的影响。方法选取105只雌性SD大鼠，根据随机数字表法将大鼠分成7组，分别为正常组、模型组、地塞米松组（阳性药物）、肺宁颗粒低剂量组、肺宁颗粒中剂量组、肺宁颗粒高剂量组、肺宁颗粒预防组，每组各15只。除去正常组，余各组均全胸接受X线单次照射15 Gy，DT30Gy／2f／1w。肺宁颗粒预防组于放射治疗前1周起开始灌胃肺宁颗粒；余组在照射后48 h起灌胃。于2、4、6周分别采用随机法每组取出5只大鼠，处死后取右肺，采用免疫组化法检测放疗后不同时段各组大鼠肺组织TGF－β1、IL－1、IL－6的含量。结果放疗后第2、4、6周模型组大鼠肺组织TGF－β1、IL－1、IL－6强表达，显著高于正常组（ P＜0．05）。经肺宁颗粒干预后各时间点大鼠肺组织中TGF－β1、IL－1、IL－6表达显著低于同时点模型组（P＜0．05），且呈一定浓度依赖性。但肺宁颗粒高、中、低剂量组TGF－β1、IL－1、IL－6表达均不同程度高于地塞米松组（P＜0．05），而肺宁颗粒预防组上述指标表达低于地塞米松组（P＜0．05）。结论肺宁颗粒能够通过降低TGF－β1、IL－1、IL－6的表达防治放射性肺损伤。肺宁颗粒各治疗组疗效不如地塞米松，但肺宁颗粒预防的疗效优于地塞米松。
목적：탐토폐저과립방치대서급성방사성폐손상（ radiation induced lung injur ，RILI）급대세포인자전화생장인자β1（transforming growth factor－β1，TGF－β1）、백개소－1（interleukin－1，IL－1）、백개소－6（interleukin－6，IL－6）적영향。방법선취105지자성SD대서，근거수궤수자표법장대서분성7조，분별위정상조、모형조、지새미송조（양성약물）、폐저과립저제량조、폐저과립중제량조、폐저과립고제량조、폐저과립예방조，매조각15지。제거정상조，여각조균전흉접수X선단차조사15 Gy，DT30Gy／2f／1w。폐저과립예방조우방사치료전1주기개시관위폐저과립；여조재조사후48 h기관위。우2、4、6주분별채용수궤법매조취출5지대서，처사후취우폐，채용면역조화법검측방료후불동시단각조대서폐조직TGF－β1、IL－1、IL－6적함량。결과방료후제2、4、6주모형조대서폐조직TGF－β1、IL－1、IL－6강표체，현저고우정상조（ P＜0．05）。경폐저과립간예후각시간점대서폐조직중TGF－β1、IL－1、IL－6표체현저저우동시점모형조（P＜0．05），차정일정농도의뢰성。단폐저과립고、중、저제량조TGF－β1、IL－1、IL－6표체균불동정도고우지새미송조（P＜0．05），이폐저과립예방조상술지표표체저우지새미송조（P＜0．05）。결론폐저과립능구통과강저TGF－β1、IL－1、IL－6적표체방치방사성폐손상。폐저과립각치료조료효불여지새미송，단폐저과립예방적료효우우지새미송。
Objective To investigate Feining granule on prevention and treatment of rat with radiation-induced lung injur ( RILI) and its effect on cytokine transforming growth factor-β1(TGF-β1), interleukin-1 (IL-1), interleukin-6 (IL-6).Methods 105 SD female rats were selected, according to random number table, and divided into seven groups: normal group, model group, dexamethasone group(positive drug), Feining granule low-dose group (FN low-dose group), FN medial-dose group, FN high-dose group and FN prevention group, 15 rats in each group.Except for normal group, all remaining groups received the X-ray irradiation of 15Gy, DT30Gy/2f/1w.FN prevention group were intragastric infused with FN granule one week before irradiation, and the other groups 48 h after irradiation.Five rats were sacrificed randomly at 2, 4, 6 weeks respectively, and right lung tissues were taken out.The contents of TGF-β1, IL-1 and IL-6 were detected by immunohistochemical method.ResuIts TGF-β1, IL-1 and IL-6 contents in lung tissue of model group at 2, 4, 6 weeks were higher than those of normal group (P<0.05).The above indicators after treated by Feining granule were lower than those of model group at each time (P<0.05),with a concentration-dependence manner to some extent.The above indicators in FN high-, medial-and low-dose group were higher than those in dexamethasone group (P<0.05), to some extent.However, the above indicators in FN prevention group were lower than those in dexamethasone group ( P<0.05 ) .ConcIusion Feining granule could prevent and cure radiation induced lung injury through decreasing TGF-β1, IL-1 and IL-6 content.The efficacy of dexamethasone is stronger than FN treatment groups, but is weaker than FN prevention.