CAJ | 학술논문

目的：探讨兔脂肪干细胞在体直接修复陈旧性全层兔膝关节软骨缺损的可行性。方法分离培养新西兰大白兔的脂肪干细胞，测定该细胞生长曲线，评价其增殖能力。每只实验兔双膝各做一个缺损，随机分为试验组和对照组，试验组缺损区填充脂肪干细胞-纤维蛋白混合物，对照组仅填充纤维蛋白。于术后第6周和第12周取材，行大体观察和组织学评价。结果冻存前后脂肪干细胞均具有较好的增殖能力。对于陈旧性关节软骨损伤，不同时间点的试验组治疗效果均好于对照组。结论脂肪干细胞增殖能力强，在体内能直接修复陈旧性全层关节软骨缺损。
목적：탐토토지방간세포재체직접수복진구성전층토슬관절연골결손적가행성。방법분리배양신서란대백토적지방간세포，측정해세포생장곡선，평개기증식능력。매지실험토쌍슬각주일개결손，수궤분위시험조화대조조，시험조결손구전충지방간세포-섬유단백혼합물，대조조부전충섬유단백。우술후제6주화제12주취재，행대체관찰화조직학평개。결과동존전후지방간세포균구유교호적증식능력。대우진구성관절연골손상，불동시간점적시험조치료효과균호우대조조。결론지방간세포증식능력강，재체내능직접수복진구성전층관절연골결손。
Objective To investigate the feasibility of adipose-derived stem cells ( ADSCs ) to repair old defects of rabbit articular cartilage in vivo. Methods Isolation and expansion of ADSCs were manipulated and the proliferation of ADSCs was detected by their growth curves before and after cryopreservation. An old osteochondral defect was crea-ted respectively in each femoral condyle of every laboratorial rabbit. These defects were divided randomly into two groups:test group and control group. The test defects were filled with ADSCs with fibrin scaffold and the control de-fects were filled with fibrin scaffold alone. Specimens were harvested at 6 and 12 weeks postoperatively for gross ob-servation and histological testing. Results The high proliferation rate of ADSCs were confirmed and the effect of test group was better than that of control group at each time points. Conclusions ADSCs possesses extensive proliferative capacity. And the transplantation of uninduced ADSCs directly can repair damaged articular cartilage in vivo.