CAJ | 학술논문

采用生长速率法测定苍术和乌药2种中药植物甲醇提取物对10种植物病原真菌的抑菌活性，为其在农药领域的开发利用提供依据。结果表明，苍术甲醇提取物对玉米大斑病菌、金橘砂皮病菌、甘蔗凤梨病菌、茶轮斑病菌、烟草黑胫病菌有很高的抑菌活性，质量浓度为10 g／L时，抑菌率在85．17％～100％，其对玉米大斑病菌、甘蔗凤梨病菌、茶轮斑病菌、水稻胡麻叶斑病菌、辣椒炭疽病菌、烟草黑胫病菌菌丝的有效中浓度（ EC50值）为0．1316～0．9802 g／L。乌药甲醇提取物对10种植物病原真菌均有很高的抑菌活性，质量浓度为10 g／L时，抑菌率均在80％以上，其中对除贡柑链格孢菌外9种植物病原真菌菌丝的EC50值为0．1020～0．6329 g／L。采用液－液萃取法和固－液萃取法分别对苍术和乌药的抑菌活性成分进行初步分离，结合活性跟踪，发现苍术抑菌活性成分主要存在于乙酸乙酯萃取层和石油醚萃取层中，乌药抑菌活性成分主要存在于乙酸乙酯萃取层中。
채용생장속솔법측정창술화오약2충중약식물갑순제취물대10충식물병원진균적억균활성，위기재농약영역적개발이용제공의거。결과표명，창술갑순제취물대옥미대반병균、금귤사피병균、감자봉리병균、다륜반병균、연초흑경병균유흔고적억균활성，질량농도위10 g／L시，억균솔재85．17％～100％，기대옥미대반병균、감자봉리병균、다륜반병균、수도호마협반병균、랄초탄저병균、연초흑경병균균사적유효중농도（ EC50치）위0．1316～0．9802 g／L。오약갑순제취물대10충식물병원진균균유흔고적억균활성，질량농도위10 g／L시，억균솔균재80％이상，기중대제공감련격포균외9충식물병원진균균사적EC50치위0．1020～0．6329 g／L。채용액－액췌취법화고－액췌취법분별대창술화오약적억균활성성분진행초보분리，결합활성근종，발현창술억균활성성분주요존재우을산을지췌취층화석유미췌취층중，오약억균활성성분주요존재우을산을지췌취층중。
A growth rate method was used for determining the antifungal activity of methanol extracts from two species of Chinese herbal medicine against ten species of plant pathogenic fungi.The results showed that the methanol extract from Atractylodes lancea ( Thunb.) DC.had a high inhibitory activity against Exserohilum turcicum ( Pass ) Leonard et Suggs, Diaporthe citri ( Fawcett ) Wolf, Ceratocystis paradoxa (Dode)Moreau,Pestalotiopsis theae(Sawada)Steyaert,Phytophthora parasitica var.Nicotianae(Breda de Hean)Tucker,with the inhibition rates of 85.17%—100%at 10 g/L.EC50 values of the methanol extract from Atractylodes lancea(Thunb.)DC.against Exserohilum turcicum(Pass)Leonard et Suggs,Ceratocystis paradoxa(Dode)Moreau,Pestalotiopsis theae(Sawada)Steyaert,Cochliobolus miyabeanus(Ito et Kubibay) Drechsler et Dastur,Colletotrichum capsici( syd.) Butl,Phytophthora parasitica var.Nicotianae( Breda de Hean) Tucker were 0.131 6—0.980 2 g/L.The methanol extract from Lindera aggregata ( Sims ) Kosterm.had a high inhibitory activity against ten species of plant pathogenic fungi, with the inhibition rates of more than 80% at 10 g/L, and with the EC50 values of 0.102 0—0.632 9 g/L against nine species among them except Alternaria citri.The active ingredients of Atractylodes lancea( Thunb.) DC.and Lindera aggregata( Sims) Kosterm.were preliminarily partitioned respectively by liquid-liquid and solid-liquid extraction and activity-directed isolation methods, and the results indicated that the active ingredients of the former mainly consisted in the ethyl acetate and petroleum ether fraction,and the latter’s in the ethyl acetate fraction.