CAJ | 학술논문

目的：研究CD47和钙网织蛋白（CRT）在浸润性乳腺导管癌（IDCAs）组织中的表达及与临床病理特征、预后的关系。方法应用免疫组化方法（ICH）对128例IDCAs及其癌旁组织中CD47、CRT的表达进行检测，并分析其与临床病理指标以及预后的关系。结果癌组织中CD47的表达为81／128，显著低于癌旁组织表达的106／128（χ2＝12．400，P＜0．05）；CRT的表达为50／128，高于癌旁组织的31／128（χ2＝6．520， P＜0．05），且各分子分型间表达差异有统计学意义（χ2＝21．510，P＜0．05），基底细胞样型和Her-（2＋）／ER （-）型明显高表达。CD47的表达与肿瘤大小、分化程度、淋巴结转移相关（χ2＝11．400、4．732、5．432，均P＜0．05）；而CRT的表达与肿瘤分化程度、肿瘤远处转移相关（χ2＝10．810、6．770，均P＜0．05）。在完成随访的98例患者中，5年生存率为68．37％。单因素生存分析显示CD47、CRT高表达组和低表达组在5年内生存率差异有统计学意义（χ2＝5．231、13．069，均P＜0．05），表达越高患者5年内生存率越低。结论 CD47、CRT与IDCAs预后差的指标相关，与5年内生存率显著相关，联合检测CD47和CRT对判断乳腺癌的预后有指导价值。
목적：연구CD47화개망직단백（CRT）재침윤성유선도관암（IDCAs）조직중적표체급여림상병리특정、예후적관계。방법응용면역조화방법（ICH）대128례IDCAs급기암방조직중CD47、CRT적표체진행검측，병분석기여림상병리지표이급예후적관계。결과암조직중CD47적표체위81／128，현저저우암방조직표체적106／128（χ2＝12．400，P＜0．05）；CRT적표체위50／128，고우암방조직적31／128（χ2＝6．520， P＜0．05），차각분자분형간표체차이유통계학의의（χ2＝21．510，P＜0．05），기저세포양형화Her-（2＋）／ER （-）형명현고표체。CD47적표체여종류대소、분화정도、림파결전이상관（χ2＝11．400、4．732、5．432，균P＜0．05）；이CRT적표체여종류분화정도、종류원처전이상관（χ2＝10．810、6．770，균P＜0．05）。재완성수방적98례환자중，5년생존솔위68．37％。단인소생존분석현시CD47、CRT고표체조화저표체조재5년내생존솔차이유통계학의의（χ2＝5．231、13．069，균P＜0．05），표체월고환자5년내생존솔월저。결론 CD47、CRT여IDCAs예후차적지표상관，여5년내생존솔현저상관，연합검측CD47화CRT대판단유선암적예후유지도개치。
Objective To evaluate the expression of CD47 and calreticulin and their relationships with clini-copathologic features,prognosis in infiltrating ductal breast carcinomas.Methods The expression of CD47 and calret-iculin was evaluated in 128 cases with infiltrating ductal breast carcinomas by means of immunohistochemistry,and the relationship between CD47 and other clinicopathologic factors as well as prognosis were analyzed.Results The expression level of CD47 in cancer nest was 81/128,which was lower than 106/128 in its adjacent normal epithelium (χ2 =12.400,P<0.05),but calreticulin expression was 50/128 and was higher in cancer nest(χ2 =21.510,P<0.05 ).In addition,the expression level of calreticulin had certain difference in different molecular subtype (χ2 =21.510,P<0.05),and an increased expression in Basal-like and Her-(2 +)/ER(-)subtypes.The expression level of CD47 was associated with tumor sizes,histological tumor grade and lymph node involvement(χ2 =11.400, 4.732,5.432,all P<0.05),and the expression level of calreticulin was associated with histological tumor grade and distant metastasis(χ2 =10.810,6.770,all P<0.05).The 5 -year survival rate of patients performed following-up was 68.37%.Univariate analysis indicated the survival rate in 5 years of high level CD47,calreticulin expression was higher than that of low level CD47,calreticulin expression(χ2 =5.231,13.069,all P<0.05).Conclusion The expression of CD47,calreticulin is associated with index of dysprognosis and survival rate in 5 years,so combined CD47 and calreticulin detect may be of prognostic value in patients with breast caner.