CAJ | 학술논문

目的：探讨自体造血干细胞（autologous peripheral blood stem cell ，APBSC）移植对恶性淋巴瘤患者血清微量元素及乳酸脱氢酶活性影响的研究。方法收集海南省人民医院2013年5月～2014年5月收治的恶性淋巴瘤患者80例，随机对照组和实验组，每组各40例，2组霍奇金淋巴瘤（ Hodgkin lymphoma ，HL）患者采用ABVD（多柔比星、博来霉素、长春新碱、达卡巴嗪）方案为主的化疗；非霍奇金淋巴瘤（ non－Hodgkinlymphoma ，NHL）患者多采用标准CHOP（环磷酰胺、长春新碱、表柔比星、地塞米松）方案为基础的化疗，采用化疗药物联合粒细胞集落刺激因子（ G－CSF）开始APBSC动员，化疗后骨髓抑制期应用10μg／（ kg? d）的G－CSF 5，检测白细胞升至3．0×109／L，血小板升至50×109／L时，采集APBSC，加入细胞冻存保护液冻存。预处理结束24 h后，将冻存的外周血造血干细胞锁骨下静脉回输给实验组患者，接受自体造血干细胞移植治疗。对照组患者继续接受化疗治疗。治疗结束后，对所有患者的Cu水平、Zn水平及乳酸脱氢酶活性进行检测。结果治疗后，2组患者的Zn水平上升（P＜0．05），Cu与Cu／Zn水平下降（P＜0．05）；与对照组相比，实验组治疗后Zn水平较高（P＜0．05），Cu与Cu／Zn水平较低（P＜0．05）；治疗后，2组患者乳酸脱氢酶活性降低（P＜0．05），与对照组比较，实验组治疗后乳酸脱氢酶活性较低（P＜0．05）。结论自体造血干细胞移植可以显著降低恶性淋巴瘤患者的Cu水平、升高Zn水平及降低乳酸脱氢酶活性，对临床有指导意义。
목적：탐토자체조혈간세포（autologous peripheral blood stem cell ，APBSC）이식대악성림파류환자혈청미량원소급유산탈경매활성영향적연구。방법수집해남성인민의원2013년5월～2014년5월수치적악성림파류환자80례，수궤대조조화실험조，매조각40례，2조곽기금림파류（ Hodgkin lymphoma ，HL）환자채용ABVD（다유비성、박래매소、장춘신감、체잡파진）방안위주적화료；비곽기금림파류（ non－Hodgkinlymphoma ，NHL）환자다채용표준CHOP（배린선알、장춘신감、표유비성、지새미송）방안위기출적화료，채용화료약물연합립세포집락자격인자（ G－CSF）개시APBSC동원，화료후골수억제기응용10μg／（ kg? d）적G－CSF 5，검측백세포승지3．0×109／L，혈소판승지50×109／L시，채집APBSC，가입세포동존보호액동존。예처리결속24 h후，장동존적외주혈조혈간세포쇄골하정맥회수급실험조환자，접수자체조혈간세포이식치료。대조조환자계속접수화료치료。치료결속후，대소유환자적Cu수평、Zn수평급유산탈경매활성진행검측。결과치료후，2조환자적Zn수평상승（P＜0．05），Cu여Cu／Zn수평하강（P＜0．05）；여대조조상비，실험조치료후Zn수평교고（P＜0．05），Cu여Cu／Zn수평교저（P＜0．05）；치료후，2조환자유산탈경매활성강저（P＜0．05），여대조조비교，실험조치료후유산탈경매활성교저（P＜0．05）。결론자체조혈간세포이식가이현저강저악성림파류환자적Cu수평、승고Zn수평급강저유산탈경매활성，대림상유지도의의。
Objective To investigate effect of autologous hematopoietic stem cell transplantation on serum trace elements and lactate dehydrogenase activity in patients with malignant lymphoma.Methods 80 patients with malignant lymphoma collected in our hospital from May 2013 to May 2014,40 cases in each group, Hodgkin lymphoma ( HL) patients were treated with ABVD ( doxorubicin, bleomycin, vincristine, dacarbazine) regimen of chemotherapy; Non-Hodgkin,s lymphoma ( NHL) patients were treated with standard CHOP ( cyclophosphamide, vincristine, epirubicin, dexamethasone) regimen of chemotherapy of two group.Chemotherapy drugs combined with granulocyte colony stimulating factor ( G-CSF) was used to mobilize APBSC.Application of 10μg/(kg? d)G-CSF 5 in bone marrow suppression after chemotherapy, detected white cell increased 3.0 ×109/L , platelets rose to 50 ×109/L, collected APBSC,The frozen stock solution of cell was joined and frozen.24 h after pre-treatment, the frozen peripheral blood hematopoietic stem cells of the subclavian vein was transfused for patients in experimental group.Control group continued to receive chemotherapy. After treatment, the Cu level, Zn level and lactate dehydrogenase activity were detected in all patients.ResuIts After treatment, Zn levels of two groups increased (P<0.05), Cu and Cu/Zn levels decreased (P<0.05).Compared with control group, the level of Zn was higher in experimental group (P<0.05), and the level of Cu and Cu/Zn was lower (P<0.05).After treatment, the lactate dehydrogenase activity of two groups decreased (P<0.05), and experimental group was more lower than control groupy (P<0.05).ConcIusion Autologous hematopoietic stem cell transplantation can significantly reduce the level of Cu in patients with malignant lymphoma , elevate Zn level and decrease the activity of lactate dehydrogenase, which has guiding significance for clinical.