CAJ | 학술논문

目的：探讨Gab2过表达对人结直肠癌细胞株SW480增殖和迁移能力的影响。方法：用携带Gab2基因的重组慢病毒颗粒（ LV-Gab2-GFP）感染人结直肠癌SW480细胞株，作为实验组（ LV-Gab2-GFP 组）；以对照慢病毒颗粒（ LV-GFP）感染人结直肠癌SW480细胞株，作为阴性对照组（ LV-GFP组）；空白对照组常规培养，不作任何处理。用RT-PCR和Western blot法分别检测细胞中Gab2 mRNA和蛋白表达的水平；CCK-8法和平板克隆实验检测细胞增殖能力的变化；划痕愈合实验检测细胞迁移能力的变化。结果：RT-PCR和Western blot均显示LV-Gab2-GFP组Gab2的表达均较对照组显著增高；与对照组比较，Gab2过表达显著增强人结直肠癌SW480细胞的增殖和迁移能力。结论：Gab2过表达可以促进SW480细胞增殖和迁移能力。
목적：탐토Gab2과표체대인결직장암세포주SW480증식화천이능력적영향。방법：용휴대Gab2기인적중조만병독과립（ LV-Gab2-GFP）감염인결직장암SW480세포주，작위실험조（ LV-Gab2-GFP 조）；이대조만병독과립（ LV-GFP）감염인결직장암SW480세포주，작위음성대조조（ LV-GFP조）；공백대조조상규배양，불작임하처리。용RT-PCR화Western blot법분별검측세포중Gab2 mRNA화단백표체적수평；CCK-8법화평판극륭실험검측세포증식능력적변화；화흔유합실험검측세포천이능력적변화。결과：RT-PCR화Western blot균현시LV-Gab2-GFP조Gab2적표체균교대조조현저증고；여대조조비교，Gab2과표체현저증강인결직장암SW480세포적증식화천이능력。결론：Gab2과표체가이촉진SW480세포증식화천이능력。
Objective:To investigate the effects of Gab2 overexpression on the proliferation and migration of human colorectal cancer cell line SW480.Methods: The experimental group (LV-Gab2-GFP group),colorectal cancer SW480 cells were transfected with recombinant lentivirus vector (LV-Gab2-GFP),the negative control group was transfected with negative control lentiviral vector ( LV-GFP) ,and the blank control group without any treatment.The mRNA and protein expression of Gab 2 in cells were identified by RT-PCR and Western blot respectively.Proliferation of the cells was detected by CCK-8 colorimeter and colony forming assay.Wound-healing assay was used to determine the cells migration .Results: RT-PCR and Western blot demonstrated that Gab 2 mRNA and protein expression significantly increased in LV-Gab2-GFP group compared with control groups;overexpression of Gab2 markedly enhanced human colorectal cancer SW 480 cells proliferation and migration compared with control groups .Conclusion:Overexpression of Gab2 accelerates human colorectal cancer SW 480 cells proliferation and migration.