中国免疫学杂志
中國免疫學雜誌
중국면역학잡지
CHINESE JOURNAL OF IMMUNOLOGY
2015年
9期
1206-1209
,共4页
方琳%王轶楠%葛敬岩%刘海岩%李晨光%李继茹%柳忠辉
方琳%王軼楠%葛敬巖%劉海巖%李晨光%李繼茹%柳忠輝
방림%왕질남%갈경암%류해암%리신광%리계여%류충휘
激活素A%神经生长因子%神经突起%钙基因相关肽
激活素A%神經生長因子%神經突起%鈣基因相關肽
격활소A%신경생장인자%신경돌기%개기인상관태
Activin A%Nerve growth factor ( NGF)%Neurite outgrowth%CGRP
目的:探讨激活素A(Activin A)与神经生长因子(Nerve growth factor,NGF)共同刺激鸡胚背根神经节(DRG)神经突起生长作用。方法:采用8 d的鸡胚DRG原代培养法,通过Activin A与NGF联合刺激,观察DRG神经突起生长和DRG神经元存活情况。采用RT-PCR检测钙基因相关肽( CGRP)。结果:Activin A与NGF体外联合培养3 d时DRG神经突起生长比单纯NGF组更明显,DRG神经元存活数量也明显增加,Activin A与NGF联合作用可以明显促进CGRP mRNA表达。结论:Activin A对NGF诱导的鸡胚DRG神经突起生长和维持DRG神经元存活具有增强作用,提示二者的联合应用可能为治疗神经元损伤及变性疾病的应用提供了新的数据和实验依据。
目的:探討激活素A(Activin A)與神經生長因子(Nerve growth factor,NGF)共同刺激鷄胚揹根神經節(DRG)神經突起生長作用。方法:採用8 d的鷄胚DRG原代培養法,通過Activin A與NGF聯閤刺激,觀察DRG神經突起生長和DRG神經元存活情況。採用RT-PCR檢測鈣基因相關肽( CGRP)。結果:Activin A與NGF體外聯閤培養3 d時DRG神經突起生長比單純NGF組更明顯,DRG神經元存活數量也明顯增加,Activin A與NGF聯閤作用可以明顯促進CGRP mRNA錶達。結論:Activin A對NGF誘導的鷄胚DRG神經突起生長和維持DRG神經元存活具有增彊作用,提示二者的聯閤應用可能為治療神經元損傷及變性疾病的應用提供瞭新的數據和實驗依據。
목적:탐토격활소A(Activin A)여신경생장인자(Nerve growth factor,NGF)공동자격계배배근신경절(DRG)신경돌기생장작용。방법:채용8 d적계배DRG원대배양법,통과Activin A여NGF연합자격,관찰DRG신경돌기생장화DRG신경원존활정황。채용RT-PCR검측개기인상관태( CGRP)。결과:Activin A여NGF체외연합배양3 d시DRG신경돌기생장비단순NGF조경명현,DRG신경원존활수량야명현증가,Activin A여NGF연합작용가이명현촉진CGRP mRNA표체。결론:Activin A대NGF유도적계배DRG신경돌기생장화유지DRG신경원존활구유증강작용,제시이자적연합응용가능위치료신경원손상급변성질병적응용제공료신적수거화실험의거。
Objective:To investigate the effect of activin A and nerve growth factor ( NGF) NGF on stimulating neurite outgrowth of dorsal root ganglia(DRG)of the embryonic chicken.Methods:In this study,we observed that activin A and NGF together induced neurite outgrowth of DRG and kept survival of DRG neurons by the primary cultured DRGs from embryonic day 8 ( E8 ) chicken.calcitonin gene-related peptide(CGRP)CGRP mRNA expressions were analyzed by RT-PCR.Results: The DRG treated with activin A +NGF had obvious neurite outgrowth ,compared with only NGF group on day 3,and the number of living DRG neurons also increased.Activin A +NGF up-regulated the mRNA expressions of CGRP in DRG.Conclusion:The Data demonstrated that activin A with NGF can synergistically stimulate DRG neurite outgrowth and maintain the DRG neurons survival , suggesting that it is more effective that NGF and activin A together treat the associated disease of nerve system.