中国免疫学杂志
中國免疫學雜誌
중국면역학잡지
CHINESE JOURNAL OF IMMUNOLOGY
2015年
9期
1257-1261
,共5页
结肠癌%血管内皮细胞生长因子%树突状细胞%相关性
結腸癌%血管內皮細胞生長因子%樹突狀細胞%相關性
결장암%혈관내피세포생장인자%수돌상세포%상관성
Colon cancer%VEGF%DCs%Correlation
目的：研究结肠癌组织VEGF表达与树突状细胞（ DCs）浸润的临床意义，并且探讨其与外周血清VEGF水平及DCs活化水平的相关性。方法：采用免疫组化法测定结肠癌和癌旁组织内VEGF和S-100蛋白的表达。采用酶联免疫吸附实验测定结肠癌患者术前及健康受试者外周血VEGF水平，分离结肠癌患者术前和健康受试者外周血单核细胞，利用TNF-α体外诱导为DCs，流式细胞仪检测DCs表面CD83的表达，分析结肠癌组织VEGF表达与DCs浸润的临床意义以及与外周血清VEGF水平及DCs活化水平的相关性。结果：①结肠癌组织VEGF阳性表达率较结肠癌旁组织显著提高（ P＜0.01）， S-100蛋白阳性表达率较结肠癌旁组织显著降低（ P＜0.01）；②结肠癌患者外周血VEGF水平较健康受试者显著升高（ P＜0.01），而外周血DCs中CD83水平较健康受试者显著降低（ P＜0.01）；③结肠癌组织VEGF和S-100蛋白的表达水平与肿瘤的分化、分期和淋巴结转移程度相关（ P＜0.01）；④结肠癌组织VEGF表达与S-100表达呈负相关（ P＜0.01）；⑤结肠癌组织VEGF表达与外周血VEGF正相关（ P＜0.01），与DCs活化水平负相关；⑥结肠癌组织DCs浸润程度与外周血VEGF负相关（ P＜0.01），与DCs活化水平正相关（ P＜0.01）。结论：结肠癌组织VEGF表达和DCs浸润共同参与结肠癌的进展，并且VEGF可抑制DCs浸润和活化。
目的：研究結腸癌組織VEGF錶達與樹突狀細胞（ DCs）浸潤的臨床意義，併且探討其與外週血清VEGF水平及DCs活化水平的相關性。方法：採用免疫組化法測定結腸癌和癌徬組織內VEGF和S-100蛋白的錶達。採用酶聯免疫吸附實驗測定結腸癌患者術前及健康受試者外週血VEGF水平，分離結腸癌患者術前和健康受試者外週血單覈細胞，利用TNF-α體外誘導為DCs，流式細胞儀檢測DCs錶麵CD83的錶達，分析結腸癌組織VEGF錶達與DCs浸潤的臨床意義以及與外週血清VEGF水平及DCs活化水平的相關性。結果：①結腸癌組織VEGF暘性錶達率較結腸癌徬組織顯著提高（ P＜0.01）， S-100蛋白暘性錶達率較結腸癌徬組織顯著降低（ P＜0.01）；②結腸癌患者外週血VEGF水平較健康受試者顯著升高（ P＜0.01），而外週血DCs中CD83水平較健康受試者顯著降低（ P＜0.01）；③結腸癌組織VEGF和S-100蛋白的錶達水平與腫瘤的分化、分期和淋巴結轉移程度相關（ P＜0.01）；④結腸癌組織VEGF錶達與S-100錶達呈負相關（ P＜0.01）；⑤結腸癌組織VEGF錶達與外週血VEGF正相關（ P＜0.01），與DCs活化水平負相關；⑥結腸癌組織DCs浸潤程度與外週血VEGF負相關（ P＜0.01），與DCs活化水平正相關（ P＜0.01）。結論：結腸癌組織VEGF錶達和DCs浸潤共同參與結腸癌的進展，併且VEGF可抑製DCs浸潤和活化。
목적：연구결장암조직VEGF표체여수돌상세포（ DCs）침윤적림상의의，병차탐토기여외주혈청VEGF수평급DCs활화수평적상관성。방법：채용면역조화법측정결장암화암방조직내VEGF화S-100단백적표체。채용매련면역흡부실험측정결장암환자술전급건강수시자외주혈VEGF수평，분리결장암환자술전화건강수시자외주혈단핵세포，이용TNF-α체외유도위DCs，류식세포의검측DCs표면CD83적표체，분석결장암조직VEGF표체여DCs침윤적림상의의이급여외주혈청VEGF수평급DCs활화수평적상관성。결과：①결장암조직VEGF양성표체솔교결장암방조직현저제고（ P＜0.01）， S-100단백양성표체솔교결장암방조직현저강저（ P＜0.01）；②결장암환자외주혈VEGF수평교건강수시자현저승고（ P＜0.01），이외주혈DCs중CD83수평교건강수시자현저강저（ P＜0.01）；③결장암조직VEGF화S-100단백적표체수평여종류적분화、분기화림파결전이정도상관（ P＜0.01）；④결장암조직VEGF표체여S-100표체정부상관（ P＜0.01）；⑤결장암조직VEGF표체여외주혈VEGF정상관（ P＜0.01），여DCs활화수평부상관；⑥결장암조직DCs침윤정도여외주혈VEGF부상관（ P＜0.01），여DCs활화수평정상관（ P＜0.01）。결론：결장암조직VEGF표체화DCs침윤공동삼여결장암적진전，병차VEGF가억제DCs침윤화활화。
Objective:To investigate the clinic significance of vascular endothelial cell growth factor ( VEGF) expression and dendritic cells ( DCs ) infiltration in colon cancer tissues , and to analysis the correlation between the sera VEGF with DCs activation.Methods:The expressions of VEGF and S-100 protein in colon carcinoma tissue and pericarcinous tissue were determined by immumohistochemistry.The serum VEGF level of colon carcinoma patients in preoperation and healthy subjects was detected using ELISA.Peripheral blood monocytes were isolated in preoperative patients and healthy subjects and induced to DCs by TNF -αin vitro.The surface expression of CD 83 in DCs was assayed with flow cytometry.Results: Expression of VEGF protein positive in colon carcinoma tissue was significantly higher than the pericarcinous tissue (P<0.01),while S-100 positive expression of colon carcinoma tissue was significantly lower than the pericarcinous tissue ( P<0.01 ).The serum VEGF level in colon carcinoma patients was significantly higher than healthy subjects ( P<0.01 ) ,while the CD83 level in peripheral blood DCs was significantly lower than healthy subjects ( P<0.01 ).The expression of VEGF and S-100 protein in colon carcinoma tissue was correlated with tumor differentiation , stage and lymph node metastasis ( P<0.01 ).The expression of VEGF was inversely correlated with S-100 ( P<0.01 ) in colon carcinoma tissue , while positively correlated with the serum VEGF ( P<0.01 ) and inversely correlated with DCs activation.The infiltration degree of DCs was inversely correlated with serum VEGF , and positively correlated with the DCs activation.Conclusion:VEGF expression in colon carcinoma tissue and DCs infiltration co-participate in the progress of colon cancer ,and VEGF could inhibit the infiltration and activation of DCs.
