中国免疫学杂志
中國免疫學雜誌
중국면역학잡지
CHINESE JOURNAL OF IMMUNOLOGY
2015年
9期
1178-1182
,共5页
谌贝贝%曹惠敏%李蓉%承欧梅
諶貝貝%曹惠敏%李蓉%承歐梅
심패패%조혜민%리용%승구매
利福平%全脑缺血%小胶质细胞%炎症反应
利福平%全腦缺血%小膠質細胞%炎癥反應
리복평%전뇌결혈%소효질세포%염증반응
Rifampicin%Global cerebral ischemia%Microglia%Inflammatory response
目的：探讨利福平对大鼠全脑缺血／再灌注损伤的保护作用及对小胶质活化的影响。方法：采用双侧颈总动脉夹闭合并低血压建立全脑缺血／再灌注大鼠模型，成年雄性SD大鼠42只，随机分成3组，假手术组（ S ），缺血再灌注组（ I／R），利福平干预组（ I／R＋RFP）。利福平（20 mg／kg）在缺血后30 min腹腔注射。采用Morris水迷宫测定大鼠认知功能改变， HE染色观察海马CA1区病理学变化，免疫组织化学方法检测海马CA1区小胶质细胞活化情况，酶联免疫法（ ELISA法）测定各组大鼠海马组织IL-1β、IL-6和TNF-α的表达水平。结果：利福平对大鼠急性全脑缺血／再灌注损伤后的行为学有明显的改善作用， I／R＋RFP组大鼠的寻台潜伏期明显缩短，同时，该组大鼠海马神经元损伤数目也显著减少。此外，利福平处理后全脑缺血／再灌注大鼠海马CA1区小胶质细胞活化明显受到抑制，海马组织内IL-1β、IL-6和TNF-α表达显著下调。结论：利福平对全脑缺血／再灌注大鼠脑损伤有明显的保护作用，其机制可能与抑制小胶质细胞活化，减少IL-1β、IL-6和TNF-α等炎症因子的释放，从而抑制炎症反应相关。
目的：探討利福平對大鼠全腦缺血／再灌註損傷的保護作用及對小膠質活化的影響。方法：採用雙側頸總動脈夾閉閤併低血壓建立全腦缺血／再灌註大鼠模型，成年雄性SD大鼠42隻，隨機分成3組，假手術組（ S ），缺血再灌註組（ I／R），利福平榦預組（ I／R＋RFP）。利福平（20 mg／kg）在缺血後30 min腹腔註射。採用Morris水迷宮測定大鼠認知功能改變， HE染色觀察海馬CA1區病理學變化，免疫組織化學方法檢測海馬CA1區小膠質細胞活化情況，酶聯免疫法（ ELISA法）測定各組大鼠海馬組織IL-1β、IL-6和TNF-α的錶達水平。結果：利福平對大鼠急性全腦缺血／再灌註損傷後的行為學有明顯的改善作用， I／R＋RFP組大鼠的尋檯潛伏期明顯縮短，同時，該組大鼠海馬神經元損傷數目也顯著減少。此外，利福平處理後全腦缺血／再灌註大鼠海馬CA1區小膠質細胞活化明顯受到抑製，海馬組織內IL-1β、IL-6和TNF-α錶達顯著下調。結論：利福平對全腦缺血／再灌註大鼠腦損傷有明顯的保護作用，其機製可能與抑製小膠質細胞活化，減少IL-1β、IL-6和TNF-α等炎癥因子的釋放，從而抑製炎癥反應相關。
목적：탐토리복평대대서전뇌결혈／재관주손상적보호작용급대소효질활화적영향。방법：채용쌍측경총동맥협폐합병저혈압건립전뇌결혈／재관주대서모형，성년웅성SD대서42지，수궤분성3조，가수술조（ S ），결혈재관주조（ I／R），리복평간예조（ I／R＋RFP）。리복평（20 mg／kg）재결혈후30 min복강주사。채용Morris수미궁측정대서인지공능개변， HE염색관찰해마CA1구병이학변화，면역조직화학방법검측해마CA1구소효질세포활화정황，매련면역법（ ELISA법）측정각조대서해마조직IL-1β、IL-6화TNF-α적표체수평。결과：리복평대대서급성전뇌결혈／재관주손상후적행위학유명현적개선작용， I／R＋RFP조대서적심태잠복기명현축단，동시，해조대서해마신경원손상수목야현저감소。차외，리복평처리후전뇌결혈／재관주대서해마CA1구소효질세포활화명현수도억제，해마조직내IL-1β、IL-6화TNF-α표체현저하조。결론：리복평대전뇌결혈／재관주대서뇌손상유명현적보호작용，기궤제가능여억제소효질세포활화，감소IL-1β、IL-6화TNF-α등염증인자적석방，종이억제염증반응상관。
Objective:To investigate the protective effect of rifampicin in a rat model of global cerebral ischemia /reperfusion ( GCIR) and discuss the influence of rifampicin on microglial activation.Methods:The GCIR rat model was induced via the bilateral occlusion of the common carotid arteries and systemic hypotension.Forty-two male SD (Sprague-Dawley) rats were randomly assigned to three groups:sham group ,I/R and I/R+FRP treated group.The rats in I/R+RFP group were treated with rifampicin 20 mg/kg by intra-peritoneal injection 30 min after reperfusion , while the other groups were treated with normal saline.Morris water maze test was performed for neurobehavioral test ,HE staining was detected for pathomorphology changes of neurons in CA 1 region.Microglia was im-munohistochemically stained in CA 1 region using ionized calcium adaptive molecular 1 ( IBA-1) as the marker.The protein levels of IL-1β,IL-6 and TNF-αin the hippocampal tissues of rats were also measured by enzyme-linked immunosorbent assay.Results:Rifampin improved the behavior ,shorten the escape latency of rats following GCIR obviously ( P<0.05 ) and reduced the neuron damage in hipp-ocampal CA1 region of rats after GCIR (P<0.05).Additionally,in I/R+FRP treated group the activation of microglia also showed a significantly inhibited compared with I/R group(P<0.05).Futhermore,we also found the expression of IL-1β,IL-6 and TNF-αin hipp-ocampal reduced obviously in I/R+FRP group ( P<0.05 ).Conclusion: Rifampin have obvious protective effect in the rat model of GCIR.The underlying mechanism may be associated with inhibition the activation of microglia ,reduction the expression of IL-1β,IL-6 and TNF-αand suppression the inflammatory response finally.
