CAJ | 학술논문

目的：探讨microRNA-199a／b-3p抑制乳腺癌细胞运动能力的机制。方法：在乳腺癌MDA-MB-231细胞中，转入miR-199a／b-3p mimcs，免疫印迹法检测PAK4的表达量变化；迁移和侵袭实验检测，超表达miR-199a／b-3p和干扰PAK4表达对乳腺癌迁移侵袭的影响；细胞膜皱起检测，超表达miR-199a／b-3p和干扰PAK4表达对乳腺癌细胞细胞膜皱起的影响。结果：在MDA-MB-231细胞中，超表达miR-199a／b-3p或干涉内源PAK4蛋白表达，均能下调细胞的迁移率、侵袭率和平均细胞膜皱起面积。结论：miR-199a／b-3p抑制乳腺癌细胞运动能力部分是通过抑制PAK4表达实现的，且miR-199a／b-3p具有抗乳腺癌迁移药物开发的潜质。
목적：탐토microRNA-199a／b-3p억제유선암세포운동능력적궤제。방법：재유선암MDA-MB-231세포중，전입miR-199a／b-3p mimcs，면역인적법검측PAK4적표체량변화；천이화침습실험검측，초표체miR-199a／b-3p화간우PAK4표체대유선암천이침습적영향；세포막추기검측，초표체miR-199a／b-3p화간우PAK4표체대유선암세포세포막추기적영향。결과：재MDA-MB-231세포중，초표체miR-199a／b-3p혹간섭내원PAK4단백표체，균능하조세포적천이솔、침습솔화평균세포막추기면적。결론：miR-199a／b-3p억제유선암세포운동능력부분시통과억제PAK4표체실현적，차miR-199a／b-3p구유항유선암천이약물개발적잠질。
Objective:To analyze the inhibiting mechanism of microRNA-199a/b-3p ( miR-199a/b-3p) on cell motility of breast cancer cells.Methods:The expression of PAK4 in MDA-MB-231 cells transfected with miR-199a/b-3p was detected by Western blot.The biology behaviors of MDA-MB-231 cells transfected with miR-199a/b-3p or PAK4 SiRNA were analysed by cell migration assay,invasion assay and protrusion dynamics.Results: MiR-199a/b-3p could suppress the expression of PAK 4 in MDA-MB-231 cells.Comparing with normal control ,miR-199a/b-3p or PAK4 SiRNA could suppress the migration ,invasion and membrane protrusion of MDA-MB-231 cells.Conclusion:miR-199a/b-3p could suppress the expression of PAK4,which are considered key breast cancer suppressors and inhibit the cell motility of breast cancer cells.