CAJ | 학술논문

克隆得一株新分离病毒草鱼呼肠孤病毒（GCRV）096的长为1981 bp 的 vp4基因，分析该基因的生物信息及其编码蛋白的特性。生物信息学分析表明，同一基因型 GCRV 分离株 vp4基因间的差异不大，但不同基因型GCRV 分离株 vp4基因间却存在很大的差异。GCRV 096 VP4蛋白含有多个功能位点、2个跨膜结构域及多个潜在的抗原决定簇。构建 pEGFP-N3-VP4真核表达质粒，并转染至草鱼肾（Ctenopharyngodon idellus kidney, CIK）细胞中，经荧光显微镜观察和 Western blot 鉴定，表明 GCRV 096 VP4融合蛋白在 CIK 细胞中得以成功表达。
극륭득일주신분리병독초어호장고병독（GCRV）096적장위1981 bp 적 vp4기인，분석해기인적생물신식급기편마단백적특성。생물신식학분석표명，동일기인형 GCRV 분리주 vp4기인간적차이불대，단불동기인형GCRV 분리주 vp4기인간각존재흔대적차이。GCRV 096 VP4단백함유다개공능위점、2개과막결구역급다개잠재적항원결정족。구건 pEGFP-N3-VP4진핵표체질립，병전염지초어신（Ctenopharyngodon idellus kidney, CIK）세포중，경형광현미경관찰화 Western blot 감정，표명 GCRV 096 VP4융합단백재 CIK 세포중득이성공표체。
GCRV 096 is a new grass carp reovirus strain. The vp4 gene 1 981 bp in length in GCRV 096 is cloned, and the characteristics of the vp4 genes and the VP4 proteins in GCRVs are analyzed. Results of bioinformatics analysis show that there is little difference between the vp4 genes in GCRV strains attributed to the same genotype, but the difference between vp4 genes in GCRV strains attributed to different genotypes is much more. The VP4 protein contains multiple functional sites, two transmembrane domain and many potential antigenic eukaryotic determinants. Furthermore, the expression plasmid pEGFP-N3-VP4 of the vp4 gene in GCRV 096 is constructed and transformed into Ctenopharyngodon idellus kidney (CIK) cells. Fluorescence microscope and western blot results indicate that the VP4 fusion protein is successfully expressed in CIK cells.