CAJ | 학술논문

目的：研究miR-421在胃癌BGC-823细胞凋亡过程中的分子机制。方法通过siRNA转染技术将miR-421 mimics与miR-421 inhibitors转染BGC-823胃癌细胞；Western blotting方法分析细胞凋亡相关蛋白caspase-3、Bax、Bcl-2以及细胞凋亡受体蛋白TNFR-Ⅰ、TNFR-Ⅱ的表达水平。结果 miR-421 mimics与miR-421 inhibitors成功转染BGC-823胃癌细胞；caspase-3、Bax蛋白在miR-421 inhibitors转染细胞中表达增强，而Bcl-2、TNFR-Ⅰ与TNFR-Ⅱ则表达降低。结论 miR-421在BGC-823胃癌细胞中可能通过调控肿瘤细胞凋亡相关蛋白caspase-3、Bax、Bcl-2的表达及肿瘤细胞表面凋亡受体蛋白TNFR的表达从而影响BGC-823的凋亡。
목적：연구miR-421재위암BGC-823세포조망과정중적분자궤제。방법통과siRNA전염기술장miR-421 mimics여miR-421 inhibitors전염BGC-823위암세포；Western blotting방법분석세포조망상관단백caspase-3、Bax、Bcl-2이급세포조망수체단백TNFR-Ⅰ、TNFR-Ⅱ적표체수평。결과 miR-421 mimics여miR-421 inhibitors성공전염BGC-823위암세포；caspase-3、Bax단백재miR-421 inhibitors전염세포중표체증강，이Bcl-2、TNFR-Ⅰ여TNFR-Ⅱ칙표체강저。결론 miR-421재BGC-823위암세포중가능통과조공종류세포조망상관단백caspase-3、Bax、Bcl-2적표체급종류세포표면조망수체단백TNFR적표체종이영향BGC-823적조망。
Objective To study the molecular mechanism of miR-421 in the apoptosis of BGC-823 gas-tric cancer cells. Methods miR-421 mimics and miR-421 inhibitors were transfected into BGC-823 cells by siRNA transfection technology. The expression of proteins related to apoptosis or receptors such as caspase-3, Bax, Bcl-2, TNFR-Ⅰand TNFR-Ⅱwere detected by Western blotting. Results The miR-421 mimics and miR-421 inhibitors were transfected into BGC-823 cells successfully. The expression of caspase-3 and Bax proteins were en-hanced in BGC-823 cells transfected with miR-421 inhibitors, while the expression of Bcl-2, TNFR-Ⅰand TNFR-Ⅱwere decreased. Conclusion The expression of apoptosis proteins caspase-3, Bax, Bcl-2 and cell surface recep-tor proteins TNFR are regulated by miRNA-421 to influence the apoptosis of BGCe-823 gastric cancer cells.