CAJ | 학술논문

目的：研究miR-7（MicroRNA-7，miRNA-7）对小鼠肠系膜淋巴结αβT淋巴细胞比例的影响并初步探讨其意义。方法：利用miR-7基因敲减（ Knockdown ，KD）小鼠模型，观察其肠系膜淋巴结大小、重量和淋巴结细胞总数，以及HE染色的病理学变化；流式细胞术（ FACS）检测淋巴结中αβT淋巴细胞亚群（ CD3＋T细胞、CD4＋T细胞和CD8＋T细胞）以及CD4＋T细胞和CD8＋T细胞的活化相关分子CD69、CD62L和功能相关因子IFN-γ表达的情况。结果：与野生型（ Wild type，WT）小鼠相比，miR-7KD小鼠肠系膜淋巴结大小、重量指数和淋巴结细胞总数均显著增高（P＜0.05），且HE染色显示MLNs有病理性改变；肠系膜淋巴结中αβCD3＋T细胞比例增加显著，其中以CD4＋T细胞比例变化最为明显（ P＜0.05），而CD19＋B淋巴细胞比例明显下调（ P＜0.05），但各亚群细胞总数均明显增加（ P＜0.05）；最后，CD4＋T细胞和CD8＋T细胞中的CD62 L＋细胞的比例均显著下调（ P＜0.05），而CD69＋细胞和IFN-γ＋细胞比例均显著上调（ P＜0.05）。结论：miR-7敲减后可显著影响肠系膜淋巴结中αβT淋巴细胞的组成比例，提示其对肠系膜淋巴结中淋巴细胞的组成和功能具有重要的调控作用。
목적：연구miR-7（MicroRNA-7，miRNA-7）대소서장계막림파결αβT림파세포비례적영향병초보탐토기의의。방법：이용miR-7기인고감（ Knockdown ，KD）소서모형，관찰기장계막림파결대소、중량화림파결세포총수，이급HE염색적병이학변화；류식세포술（ FACS）검측림파결중αβT림파세포아군（ CD3＋T세포、CD4＋T세포화CD8＋T세포）이급CD4＋T세포화CD8＋T세포적활화상관분자CD69、CD62L화공능상관인자IFN-γ표체적정황。결과：여야생형（ Wild type，WT）소서상비，miR-7KD소서장계막림파결대소、중량지수화림파결세포총수균현저증고（P＜0.05），차HE염색현시MLNs유병이성개변；장계막림파결중αβCD3＋T세포비례증가현저，기중이CD4＋T세포비례변화최위명현（ P＜0.05），이CD19＋B림파세포비례명현하조（ P＜0.05），단각아군세포총수균명현증가（ P＜0.05）；최후，CD4＋T세포화CD8＋T세포중적CD62 L＋세포적비례균현저하조（ P＜0.05），이CD69＋세포화IFN-γ＋세포비례균현저상조（ P＜0.05）。결론：miR-7고감후가현저영향장계막림파결중αβT림파세포적조성비례，제시기대장계막림파결중림파세포적조성화공능구유중요적조공작용。
Objective:To detect the change of proportions of αβT lymphocytes in mesenteric lymph nodes of miR-7 knockdown (miR-7KD)mice,and preliminarily explore its importance.Methods: The volume,weight index and total cells number of mesenteric lymph node in miR-7KD mice were measured.The pathologic morphology change of mesenteric lymph nodes was observed by HE stai -ning.And the changes on proportion of αβT lymphocytes in mesenteric lymph nodes of miR-7KD mice were analyzed by Flow cytometry.Results:Compared with those of WT (wild type) mice,the volume,weight index,and the total cells number of mesenteric lymph node were significantly increased ( P<0.05 ).Moreover ,the pathologic morphology was significantly changed.The proportion and numbers of T lymphocyte were significantly increased;however,the proportion of B lymphocyte were significantly decreased (P<0.05). Notably,the proportion and number of CD4+T cells and CD8+T cells were significantly increased (P<0.05).Meanwhile,CD62L+T cell proportion were vigorously reduced and CD 69+T cell and IFN-γ+T cell proportion were notably up-regulated,which belong to CD4+and CD8+T cell(P<0.05).Conclusion:There was significant influence in the proportion of αβT lymphocytes in mesenteric lymph nodes of miR-7KD mice,suggesting that miR-7 might play an important role in the composition and function of lymphocytes in mesenteric lymph nodes.