CAJ | 학술논문

目的：探讨参附注射液治疗在大鼠脑缺血再灌注损伤中的保护作用及其对Nrf2信号通路的影响。方法：将68只雄性SD大鼠随机分为假手术组、脑缺血再灌注组、缺血再灌注损伤加参附注射液（8 mg／kg ）治疗组，采用线栓法制作大鼠大脑中动脉缺血再灌注损伤模型，再灌注后24 h对大鼠进行行为学评分后处死并取脑，采用免疫印迹法检测参附注射液对Nrf2、HO-1、NQO1和cleaved-caspase-3蛋白表达的影响，尼氏法检测脑缺血再灌注损伤后的神经元损伤程度。结果：参附注射液治疗可以显著增加核内Nrf2蛋白的表达（ P＜0.05），显著增加脑缺血再灌注损伤围脑组织中HO-1、NQO-1的表达量（ P＜0.05），明显减少cleaved-caspase-3蛋白的表达和受损神经元的个数，显著改善神经功能评分（ P＜0.05）。结论：参附注射液可以通过上调Nrf2信号通路从而在脑缺血再灌注损伤中发挥神经保护作用。
목적：탐토삼부주사액치료재대서뇌결혈재관주손상중적보호작용급기대Nrf2신호통로적영향。방법：장68지웅성SD대서수궤분위가수술조、뇌결혈재관주조、결혈재관주손상가삼부주사액（8 mg／kg ）치료조，채용선전법제작대서대뇌중동맥결혈재관주손상모형，재관주후24 h대대서진행행위학평분후처사병취뇌，채용면역인적법검측삼부주사액대Nrf2、HO-1、NQO1화cleaved-caspase-3단백표체적영향，니씨법검측뇌결혈재관주손상후적신경원손상정도。결과：삼부주사액치료가이현저증가핵내Nrf2단백적표체（ P＜0.05），현저증가뇌결혈재관주손상위뇌조직중HO-1、NQO-1적표체량（ P＜0.05），명현감소cleaved-caspase-3단백적표체화수손신경원적개수，현저개선신경공능평분（ P＜0.05）。결론：삼부주사액가이통과상조Nrf2신호통로종이재뇌결혈재관주손상중발휘신경보호작용。
Objective:To study the neuro-protective effects of Shenfu injection on Nrf 2 signaling pathway affected by cerebral ischemia reperfusion.Methods: A total of 68 adult male Sprague-Dawley rats were randomly divided into sham group , cerebral ischemia-reperfusion group,and(8 mg/kg)Shenfu injection treatment group.Shenfu injection was injected intraperitoneally in the rats after MCAO.Neurologic deficit was evaluated after 24 hours of reperfusion.All the rats were sacrificed after 24 h of ischemia-reoerfusion for biochemical analysis or Nissl staining.Results:Shenfu injection treatment significantly increased the expression of Nrf 2,HO-1 and NQO-1(P<0.05).Furthermore,Shenfu injection treatment significantly reduced the expression of cleaved -caspase-3 and attenuated neurological deficits after cerebral ischemia reperfusion ( P<0.05 ).Lastly, Shenfu injection could evidently alleviate the severity of neuronal degeneration ( P<0.05 ).Conclusion:Shenfu injection could confer neuroprotection after cerebral ischemia reperfusion through modulating the Nrf2 signaling pathway.