黑龙江动物繁殖
黑龍江動物繁殖
흑룡강동물번식
2015年
5期
6-10
,共5页
柴孟龙%魏巍%何长久%王军%卫喜明%张洪涛%陈洋%刘大森%吕文发%刘国世
柴孟龍%魏巍%何長久%王軍%衛喜明%張洪濤%陳洋%劉大森%呂文髮%劉國世
시맹룡%위외%하장구%왕군%위희명%장홍도%진양%류대삼%려문발%류국세
黑萨福克%超数排卵%原核胚
黑薩福剋%超數排卵%原覈胚
흑살복극%초수배란%원핵배
Black Suffolk%pronuclear embryo%superovulation
为提高黑萨福克绵羊原核期胚胎的生产效率，通过 CIDR＋FSH＋PMSG和CIDR＋FSH＋PG两种激素方案对供体进行超数排卵，将冲出的胚胎移植入同期发情处理的受体。其中CIDR＋FSH＋PMSG注射组供体平均黄体数、可用胚数和移植妊娠率均高于CIDR＋FSH＋PG 注射组（ P＜0．05）。同时，在CIDR＋FSH＋PMSG注射组，不同FSH剂量注射对黑萨福克绵羊超数排卵有影响。在180～240 IU剂量组，供体羊的平均黄体数、可用胚胎数高于另一组。繁殖季节（秋季）供体平均黄体数、可用胚胎数均显著高于春季（ P＜0．05）。
為提高黑薩福剋綿羊原覈期胚胎的生產效率，通過 CIDR＋FSH＋PMSG和CIDR＋FSH＋PG兩種激素方案對供體進行超數排卵，將遲齣的胚胎移植入同期髮情處理的受體。其中CIDR＋FSH＋PMSG註射組供體平均黃體數、可用胚數和移植妊娠率均高于CIDR＋FSH＋PG 註射組（ P＜0．05）。同時，在CIDR＋FSH＋PMSG註射組，不同FSH劑量註射對黑薩福剋綿羊超數排卵有影響。在180～240 IU劑量組，供體羊的平均黃體數、可用胚胎數高于另一組。繁殖季節（鞦季）供體平均黃體數、可用胚胎數均顯著高于春季（ P＜0．05）。
위제고흑살복극면양원핵기배태적생산효솔，통과 CIDR＋FSH＋PMSG화CIDR＋FSH＋PG량충격소방안대공체진행초수배란，장충출적배태이식입동기발정처리적수체。기중CIDR＋FSH＋PMSG주사조공체평균황체수、가용배수화이식임신솔균고우CIDR＋FSH＋PG 주사조（ P＜0．05）。동시，재CIDR＋FSH＋PMSG주사조，불동FSH제량주사대흑살복극면양초수배란유영향。재180～240 IU제량조，공체양적평균황체수、가용배태수고우령일조。번식계절（추계）공체평균황체수、가용배태수균현저고우춘계（ P＜0．05）。
The objective of this study was to improve the efficiency of Black Suffolk pronuclear -stage embryo yield to be used for DNA microinjection in a transgenesis program.The Black Suffolk sheep was superovulated by CIDR +PMSG +FSH and CIDR +FSH +PG. Then the flushed out embryo was transferred to the estrus synchronized recipient by speculum insemination+nature mating.In the CIDR+FSH+PMSG injection group, the average number of corpus luteum, available embryo and pregnancy rate from the Black Suffolk were significantly higher compared to the CIDR+FSH+PG injection group ( P<0.05 ) . Meanwhile, different dose of FSH injection had a impact on the Black Suffolk sheep superovulation.When the FSH injection dose was during 180 ~240 IU, the average number of corpus luteum and available embryo from the Black Suffolk sheep were 15.87 and 13.88, respectively.The average number of corpus luteum and available embryo were significantly higher in the breeding season ( autumn) compared to spring( P<0.05) .
