CAJ | 학술논문

背景：近年研究发现骨髓间充质干细胞经过长时间体外培养后，可自然分化为神经干细胞，从而再定向分化为神经细胞及神经胶质细胞，为帕金森病、脑梗死后遗症、小脑萎缩和脑发育不良等疾病的治疗提供了一种新的思路。 　　目的：探讨神经干细胞移植对脑出血恢复期大鼠神经功能的影响及其作用机制。 　　方法：将60只雄性SD大鼠按照随机数字表法分为正常组(18只)、脑出血组(21只)和移植组(21只)，后2组大鼠采用Ⅶ型胶原酶诱导法建立大鼠脑出血模型，造模21 d后移植组大鼠通过尾静脉注射神经干细胞，脑出血组给予等量生理盐水。移植后第7，14，21天进行改良黏附物移除试验(MST)评分，评分结束后处死各组大鼠，采用RT-PCR法测定大鼠出血周围脑组织促血管生成素1 mRNA表达，采用Western Blotting法检测大鼠出血周围脑组织酪氨酸激酶受体2蛋白表达。 　　结果与结论：与正常组相比，脑出血组和移植组各时点MST评分均明显降低，差异均有显著性意义(P <0.05)；从移植后7 d开始移植组各时点的MST评分均明显高于脑出血组，差异均有显著性意义(P <0.05)。移植后7，14，21 d移植组和脑出血组促血管生成素1 mRNA和酪氨酸激酶受体2蛋白含量均明显高于正常组，且移植组升高更为明显，差异均有显著性意义(P均<0.05)。结果表明神经干细胞移植能够有效促进脑出血恢复期大鼠神经功能的恢复，其机制可能与增加出血周围脑组织促血管生成素及酪氨酸激酶受体2的含量有关。
배경：근년연구발현골수간충질간세포경과장시간체외배양후，가자연분화위신경간세포，종이재정향분화위신경세포급신경효질세포，위파금삼병、뇌경사후유증、소뇌위축화뇌발육불량등질병적치료제공료일충신적사로。 　　목적：탐토신경간세포이식대뇌출혈회복기대서신경공능적영향급기작용궤제。 　　방법：장60지웅성SD대서안조수궤수자표법분위정상조(18지)、뇌출혈조(21지)화이식조(21지)，후2조대서채용Ⅶ형효원매유도법건립대서뇌출혈모형，조모21 d후이식조대서통과미정맥주사신경간세포，뇌출혈조급여등량생리염수。이식후제7，14，21천진행개량점부물이제시험(MST)평분，평분결속후처사각조대서，채용RT-PCR법측정대서출혈주위뇌조직촉혈관생성소1 mRNA표체，채용Western Blotting법검측대서출혈주위뇌조직락안산격매수체2단백표체。 　　결과여결론：여정상조상비，뇌출혈조화이식조각시점MST평분균명현강저，차이균유현저성의의(P <0.05)；종이식후7 d개시이식조각시점적MST평분균명현고우뇌출혈조，차이균유현저성의의(P <0.05)。이식후7，14，21 d이식조화뇌출혈조촉혈관생성소1 mRNA화락안산격매수체2단백함량균명현고우정상조，차이식조승고경위명현，차이균유현저성의의(P균<0.05)。결과표명신경간세포이식능구유효촉진뇌출혈회복기대서신경공능적회복，기궤제가능여증가출혈주위뇌조직촉혈관생성소급락안산격매수체2적함량유관。
BACKGROUND:Recent studies have found that bone marrow mesenchymal stem cels that culturedin vitro for a long time can naturaly differentiate into neural stem cels, which then differentiate into neurons and glial cels, thereby providing a new therapeutic thinking for Parkinson’s disease, sequela of cerebral infarction, cerebelar atrophy and brain dysplasia. OBJECTIVE:To discuss the influence of neural stem cel transplantation on neurologic function of rats with cerebral hemorrhage at recovery stage and the relevant mechanism of action. METHODS: Sixty male Sprague-Dawley rats were randomly divided into normal group (n=18), cerebral hemorrhage group (n=21) and transplantation group (n=21). Cerebral hemorrhage models were established in the latter two groups using VII type colagen enzyme induction method. At 21 days of modeling, rats in the transplantation group were injected neural stem cels via the tail vein, and those in the other two groups received the same volume of normal saline. At 7, 14, 21 days after cel transplantation, modified adhesive removal test (MST) was employed to evaluate the neurologic function of rats, and then the rats were kiled. RT-PCR was used to detect angiopoietin-1 mRNA expression in the bleeding tissues, and western blot assay was employed to measure tyrosine kinase receptor-2 protein expression. RESULTS AND CONCLUSION:Compared with the normal group, the MST scores in the cerebral hemorrhage group and transplantation group were significantly decreased (P< 0.05). From the 7th day after transplantation, MST scores in the transplantation group were significantly higher than those in the cerebral hemorrhage group (P < 0.05). At 7, 14, 21 days after transplantation, expressions of angiopoietin-1 mRNA and tyrosine kinase receptor-2 protein were ranked as folows: transplantation group > cerebral hemorrhage group > normal group, and there was a significant difference among the three groups (P< 0.05). These findings indicate that neural stem cel transplantation can effectively promote the neurologic recovery of rats with cerebral hemorrhage at recovery stage, and the concrete mechanism may be related to the increase of angiopoietin-1 mRNA and tyrosine kinase receptor-2 protein in the bleeding tissues.