CAJ | 학술논문

背景：肿瘤干细胞与卵巢癌的发生和发展等之间存在十分密切的联系，CD90+是一种重要的肿瘤干细胞标志物。 　　目的：探索卵巢癌细胞中CD90+肿瘤干细胞生物学特性。 　　方法：获得原代卵巢癌细胞并进行分析，对人卵巢癌细胞系SKOV3和原代卵巢癌细胞的CD133和CD90阳性率进行流式细胞术检测，分选获得 CD90+和 CD90-细胞，并通过反转录 PCR法对其干细胞和上皮间质化相关基因mRNA的相对表达情况进行检测。通过Transwel 小室侵袭试验对细胞侵袭能力进程观察，利用克隆形成试验对细胞增殖分化能力进行观察，利用悬浮成球试验对干细胞潜能进行观察。并通过免疫缺陷小鼠体内有限稀释成瘤试验，对成瘤时间以及成瘤率进行观察。 　　结果与结论：SKOV3细胞的CD133和CD90阳性率均显著低于原代卵巢癌细胞。SKOV3细胞系CD90+干细胞相关基因mRNA相对表达CD133和OCT4均显著高于SKOV3细胞系CD90-干细胞；原代卵巢癌细胞CD90+干细胞中CD44、CD133、乙醛脱氢酶1和OCT4均显著高于原代卵巢癌细胞CD90-干细胞。SKOV3细胞系CD90-和CD90+干细胞的上皮间质化相关基因mRNA相对表达水平差异有显著性意义，原代卵巢癌细胞CD90-和CD90+干细胞的上皮间质化相关基因mRNA相对表达水平差异有显著性意义。随着接种细胞数量的增加，CD90-和 CD90+细胞成瘤率呈现出不断上升的情况，成瘤时间则不断下降。其中，CD90+干细胞的上升较之 CD90-干细胞更为显著。SKOV3细胞系和原代卵巢癌细胞的 CD90+干细胞穿膜细胞数、细胞克隆数、悬浮成球数量均显著大于CD90-干细胞。提示卵巢癌细胞中CD90+干细胞可高表达干细胞相关基因和间质属性基因，并具备较高的侵袭、增殖分化能力以及成瘤能力和较大的干细胞潜能。
배경：종류간세포여란소암적발생화발전등지간존재십분밀절적련계，CD90+시일충중요적종류간세포표지물。 　　목적：탐색란소암세포중CD90+종류간세포생물학특성。 　　방법：획득원대란소암세포병진행분석，대인란소암세포계SKOV3화원대란소암세포적CD133화CD90양성솔진행류식세포술검측，분선획득 CD90+화 CD90-세포，병통과반전록 PCR법대기간세포화상피간질화상관기인mRNA적상대표체정황진행검측。통과Transwel 소실침습시험대세포침습능력진정관찰，이용극륭형성시험대세포증식분화능력진행관찰，이용현부성구시험대간세포잠능진행관찰。병통과면역결함소서체내유한희석성류시험，대성류시간이급성류솔진행관찰。 　　결과여결론：SKOV3세포적CD133화CD90양성솔균현저저우원대란소암세포。SKOV3세포계CD90+간세포상관기인mRNA상대표체CD133화OCT4균현저고우SKOV3세포계CD90-간세포；원대란소암세포CD90+간세포중CD44、CD133、을철탈경매1화OCT4균현저고우원대란소암세포CD90-간세포。SKOV3세포계CD90-화CD90+간세포적상피간질화상관기인mRNA상대표체수평차이유현저성의의，원대란소암세포CD90-화CD90+간세포적상피간질화상관기인mRNA상대표체수평차이유현저성의의。수착접충세포수량적증가，CD90-화 CD90+세포성류솔정현출불단상승적정황，성류시간칙불단하강。기중，CD90+간세포적상승교지 CD90-간세포경위현저。SKOV3세포계화원대란소암세포적 CD90+간세포천막세포수、세포극륭수、현부성구수량균현저대우CD90-간세포。제시란소암세포중CD90+간세포가고표체간세포상관기인화간질속성기인，병구비교고적침습、증식분화능력이급성류능력화교대적간세포잠능。
BACKGROUND:There is a close connection between the occurrence and development of tumor stem cels and ovarian cancer. CD90+ is an important tumor stem cel marker. OBJECTIVE: To explore the biological characteristics of CD90+ tumor stem cels in ovarian cancer cels. METHODS:The CD133 and CD90 positive rate of SKOV3 and primary ovarian cancer cels were detected by flow cytometry. The CD90+ and CD90- relative expression in stem cels and epithelium was detected by RT-PCR. Transwel invasion assay was employed to observe the cel invasion ability, clone formation test was done to observe cel proliferation and differentiation capacity, suspension bal test was adopted to observe pluripotent stem cels. The tumor formation time and tumor formation rate were observed by limited tumor dilution in immunodeficient mice. RESULTS AND CONCLUSION:The positive rates of CD133 and CD90 in SKOV3 were significantly lower than those in primary ovarian cancer cels. The expression of CD133 and OCT4 in CD90+cels of SKOVS was significantly higher than that in CD90-cels of SKOVS. The expression of CD44, CD133, acetaldehyde dehydrogenase-1 and OCT4 in CD90+stem cels of primary ovarian cancer cels was significantly higher than that in CD90-stem cels of primary ovarian cancer cels. There were significant differences in the epithelial-mesenchymal related gene expressions between CD90-and CD90+stem cels of SKOV3 and primary ovarian cancer cels. With the increase of inoculated cels, the tumor formation rate of CD90-and CD90+ cels was increased continuously, but the tumor formation time was decreased. The tumor rate of CD90-cels was lower than that of CD90+cels. The number of transmembrane cels, cel clones and suspended cel bals was significantly higher in the CD90+ stem cels than the CD90-stem cels. These findings indicate that in ovarian cancer cels, CD90+stem cels can highly express stem cel-related genes and epithelial-mesenchymal related genes, which have a higher invasion, proliferation and differentiation ability, as wel as tumorigenic and pluripotent ability.