中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2015年
32期
5215-5219
,共5页
干细胞%移植%骨髓%间充质干细胞%造血干细胞%致敏受体%流式细胞仪%致敏动物模型
榦細胞%移植%骨髓%間充質榦細胞%造血榦細胞%緻敏受體%流式細胞儀%緻敏動物模型
간세포%이식%골수%간충질간세포%조혈간세포%치민수체%류식세포의%치민동물모형
背景:造血干细胞移植对多种疾病具有治疗作用,但其取材不便,且细胞数量受年龄限制等原因,故而应用具有一定局限性。<br> 目的:探索骨髓间充质干细胞在致敏与非致敏BALB/c小鼠造血干细胞移植中的应用价值。<br> 方法:将 BALB/c 小鼠骨髓细胞在体外进行分离,采用贴壁培养的方法获得间充质干细胞,使用流式细胞仪对细胞表面的分子标记进行检测。应用异基因脾细胞输注方法建立致敏动物模型,用绿色荧光染料标记骨髓间充质干细胞,分别移植到致敏和非致敏的受体小鼠体内,并在移植后的不同时间点对间充质干细胞的归巢情况进行检测。对致敏BALB/c小鼠进行照射预处理,联合应用异基因骨髓细胞与同基因间充质干细胞移植,观察BALB/c小鼠的生存情况。<br> 结果与结论:移植48 h后,间充质干细胞在致敏受体和非致敏受体小鼠分别归巢于脾脏和骨髓。在造血干细胞的移植实验中,致敏 BALB/c 小鼠接受异基因骨髓细胞与同基因骨髓间充质干细胞联合移植,结果显示致敏BALB/c小鼠全部在移植后12-15 d死亡,生存的中位时间是14 d,而仅接受异基因骨髓细胞移植的致敏BALB/c小鼠的中位生存时间为13 d。说明细胞移植后在致敏受体内间充质干细胞主要归巢为脾脏和骨髓,联合应用间充质干细胞移植对异基因造血干/祖细胞植入致敏受体体内并没有起到有效的促进作用。
揹景:造血榦細胞移植對多種疾病具有治療作用,但其取材不便,且細胞數量受年齡限製等原因,故而應用具有一定跼限性。<br> 目的:探索骨髓間充質榦細胞在緻敏與非緻敏BALB/c小鼠造血榦細胞移植中的應用價值。<br> 方法:將 BALB/c 小鼠骨髓細胞在體外進行分離,採用貼壁培養的方法穫得間充質榦細胞,使用流式細胞儀對細胞錶麵的分子標記進行檢測。應用異基因脾細胞輸註方法建立緻敏動物模型,用綠色熒光染料標記骨髓間充質榦細胞,分彆移植到緻敏和非緻敏的受體小鼠體內,併在移植後的不同時間點對間充質榦細胞的歸巢情況進行檢測。對緻敏BALB/c小鼠進行照射預處理,聯閤應用異基因骨髓細胞與同基因間充質榦細胞移植,觀察BALB/c小鼠的生存情況。<br> 結果與結論:移植48 h後,間充質榦細胞在緻敏受體和非緻敏受體小鼠分彆歸巢于脾髒和骨髓。在造血榦細胞的移植實驗中,緻敏 BALB/c 小鼠接受異基因骨髓細胞與同基因骨髓間充質榦細胞聯閤移植,結果顯示緻敏BALB/c小鼠全部在移植後12-15 d死亡,生存的中位時間是14 d,而僅接受異基因骨髓細胞移植的緻敏BALB/c小鼠的中位生存時間為13 d。說明細胞移植後在緻敏受體內間充質榦細胞主要歸巢為脾髒和骨髓,聯閤應用間充質榦細胞移植對異基因造血榦/祖細胞植入緻敏受體體內併沒有起到有效的促進作用。
배경:조혈간세포이식대다충질병구유치료작용,단기취재불편,차세포수량수년령한제등원인,고이응용구유일정국한성。<br> 목적:탐색골수간충질간세포재치민여비치민BALB/c소서조혈간세포이식중적응용개치。<br> 방법:장 BALB/c 소서골수세포재체외진행분리,채용첩벽배양적방법획득간충질간세포,사용류식세포의대세포표면적분자표기진행검측。응용이기인비세포수주방법건립치민동물모형,용록색형광염료표기골수간충질간세포,분별이식도치민화비치민적수체소서체내,병재이식후적불동시간점대간충질간세포적귀소정황진행검측。대치민BALB/c소서진행조사예처리,연합응용이기인골수세포여동기인간충질간세포이식,관찰BALB/c소서적생존정황。<br> 결과여결론:이식48 h후,간충질간세포재치민수체화비치민수체소서분별귀소우비장화골수。재조혈간세포적이식실험중,치민 BALB/c 소서접수이기인골수세포여동기인골수간충질간세포연합이식,결과현시치민BALB/c소서전부재이식후12-15 d사망,생존적중위시간시14 d,이부접수이기인골수세포이식적치민BALB/c소서적중위생존시간위13 d。설명세포이식후재치민수체내간충질간세포주요귀소위비장화골수,연합응용간충질간세포이식대이기인조혈간/조세포식입치민수체체내병몰유기도유효적촉진작용。
BACKGROUND:Hematopoietic stem cel transplantation has therapeutic effects on many diseases, but its application has some limitations, such as cel harvesting and age-limited number of cels. <br> OBJECTIVE: To investigate the application value of bone marrow mesenchymal stem cels in hematopoietic stem cel transplantation for sensitized and non-sensitized BALB/c mice. <br> METHODS: Bone marrow cels derived from BALB/c mice were isolated and culturedin vitro to harvest mesenchymal stem cels using adherent method. The cel surface markers were detected by flow cytometry. A murine model of sensitization was established by transfusion of alogeneic spleen cels. Mesenchymal stem cels labeled with green fluorescent dye were transplanted into non-sensitized and sensitized recipient mice, and the homing of mesenchymal stem cels in vivowas monitored at different time points post transplantation. Additionaly, under irradiation pretreatment, sensitized BALB/c mice under irradiation were subjected to combined transplantation of alogeneic bone marrow cels and syngeneic mesenchymal stem cels. Survival rate of BALB/c mice was monitored daily. <br> RESULTS AND CONCLUSION: At 48 hours after transplantation, mesenchymal stem cels in sensitized and non-sensitized recipients were homing to the spleen and bone marrow, respectively. In the experiment of hematopoietic stem cel transplantation, the sensitized recipients died at 12-15 days after combined transplantation, with a median of 14 days; however, the sensitized recipients only undergoing alogeneic bone marrow cel transplantation had a survival median of 13 days. These findings indicate that the transplanted mesenchymal stem cels in sensitized recipients are mainly homing to the spleen and bone marrow, but the combination transplantation cannot enhance the transplantation of alogeneic hematopoietic stem/progenitor cels in sensitized recipients.