世界最新医学信息文摘（连续型电子期刊）
世界最新醫學信息文摘（連續型電子期刊）
세계최신의학신식문적（련속형전자기간）
World Latest Medicine Information
2015年
51期
5-6
,共2页
白细胞介素-8%基因%脓毒症
白細胞介素-8%基因%膿毒癥
백세포개소-8%기인%농독증
Interleukin IL-18%Gene%Sepsis
目的：研究白细胞介素（IL-18）基因启动子多态性位点以及相应血清IL-18含量与儿童脓毒症（Sepsis）的关系。方法双抗体夹心酶联免疫吸附实验（ELISA）法、聚合酶链反应.序列特异性引物（PCR-SSP）和测序的方法。两组间比较采用t检验，多组间比较采用方差分析，计数资料比较采用x2检验。结果①脓毒症患儿血清IL-18水平明显高于健康儿童（P<0.01），随着脓毒症患儿病情的加重，血清IL-18浓度呈上升趋势，非危重组明显高于对照组，危重组高于非危重组，极危重组血清IL-18浓度显著增高，差异有非常显著性（P<0.01），相关分析发现血IL-18水平与小儿危蕈病例评分呈负相关（P<0.01）。②脓毒症患儿-607CA基因型分布频率（76.7%）显著高于健康儿童（61.0%）， AA基因型分布频率（2.2%）显著低于健康儿童（12.2%），差异均有显著性（P<0.05）。结论脓毒症患儿血清IL-18水平明显升高，且与病情的加重有关。II-18基因启动子-607CA基因型携带者儿童更易罹患脓毒症，表明-607CA基因型可能为脓毒症易感基因型，而-607AA基因型可能对儿童患脓毒症具有对抗作用。
目的：研究白細胞介素（IL-18）基因啟動子多態性位點以及相應血清IL-18含量與兒童膿毒癥（Sepsis）的關繫。方法雙抗體夾心酶聯免疫吸附實驗（ELISA）法、聚閤酶鏈反應.序列特異性引物（PCR-SSP）和測序的方法。兩組間比較採用t檢驗，多組間比較採用方差分析，計數資料比較採用x2檢驗。結果①膿毒癥患兒血清IL-18水平明顯高于健康兒童（P<0.01），隨著膿毒癥患兒病情的加重，血清IL-18濃度呈上升趨勢，非危重組明顯高于對照組，危重組高于非危重組，極危重組血清IL-18濃度顯著增高，差異有非常顯著性（P<0.01），相關分析髮現血IL-18水平與小兒危蕈病例評分呈負相關（P<0.01）。②膿毒癥患兒-607CA基因型分佈頻率（76.7%）顯著高于健康兒童（61.0%）， AA基因型分佈頻率（2.2%）顯著低于健康兒童（12.2%），差異均有顯著性（P<0.05）。結論膿毒癥患兒血清IL-18水平明顯升高，且與病情的加重有關。II-18基因啟動子-607CA基因型攜帶者兒童更易罹患膿毒癥，錶明-607CA基因型可能為膿毒癥易感基因型，而-607AA基因型可能對兒童患膿毒癥具有對抗作用。
목적：연구백세포개소（IL-18）기인계동자다태성위점이급상응혈청IL-18함량여인동농독증（Sepsis）적관계。방법쌍항체협심매련면역흡부실험（ELISA）법、취합매련반응.서렬특이성인물（PCR-SSP）화측서적방법。량조간비교채용t검험，다조간비교채용방차분석，계수자료비교채용x2검험。결과①농독증환인혈청IL-18수평명현고우건강인동（P<0.01），수착농독증환인병정적가중，혈청IL-18농도정상승추세，비위중조명현고우대조조，위중조고우비위중조，겁위중조혈청IL-18농도현저증고，차이유비상현저성（P<0.01），상관분석발현혈IL-18수평여소인위심병례평분정부상관（P<0.01）。②농독증환인-607CA기인형분포빈솔（76.7%）현저고우건강인동（61.0%）， AA기인형분포빈솔（2.2%）현저저우건강인동（12.2%），차이균유현저성（P<0.05）。결론농독증환인혈청IL-18수평명현승고，차여병정적가중유관。II-18기인계동자-607CA기인형휴대자인동경역리환농독증，표명-607CA기인형가능위농독증역감기인형，이-607AA기인형가능대인동환농독증구유대항작용。
ABSTRACT:Objective To study relationship between IL-18 gene promoter polymorphism position and corresponding serum IL-18 level of children with sepsis. Method double antibody sandwich enzyme linked immunosorbent assay (ELISA) method, polymerase chain reaction, sequence specific primer (PCR-SSP) and sequencing method were used. Apply t test between two groups for comparison, and variance analysis for comparison between many groups, and count data comparison use x2 test. Result ① serum IL-18 level of children with sepsis were significantly higher than that of normal children (P<0.01), with aggravation of sepsis, serum concentration of IL-18 showed a rising trend, non-critical group was significantly higher than that of control group. critical group was higher than non critical group, and serum IL-18 concentration of very critical group significantly increased, difference was significant (P < 0.05). Related analysis found that serum IL-18 level and pediatric dangerous case score was negative related (P<0.01).②607CA genotype distribution frequency of children with sepsis(76.7%) was significantly higher than that of healthy children (61%), and AA genotype distribution frequency (2.2%) was significantly lower than that of healthy children (12.2%), and difference was significant (P<0.05). Conclusion serum IL-18 levels were significantly high for patients with sepsis, which was related to severity of the disease. II-18 gene promoter-607CA genotype carriers were more likely to suffer from sepsis, which suggests that-607CA genotype may be susceptible to sepsis, and-607AA genotype may have opposing role against sepsis of children.
