安徽农学通报
安徽農學通報
안휘농학통보
AUHUI AGRICULTURAL SCIENCE BULLETIN
2015年
17期
36-38
,共3页
蓝点马鲛%头肾%cDNA文库%构建
藍點馬鮫%頭腎%cDNA文庫%構建
람점마교%두신%cDNA문고%구건
Scomberomorus niphonius%Head kidney%cDNA library%Construction
研究运用SMART技术对蓝点马鲛头肾构建了cDNA文库，该文库原始文库滴度为2.26×106pfu/mL，文库容量为1.36×106pfu，符合文库构建的要求。从文库中随机挑选24个单克隆菌，以载体的通用引物进行菌落检测PCR，结果表明：重组率为95.8%，插入片段大小平均为1000bp。该文库的构建满足了基因的分离与筛选，为进一步研究其相关基因克隆及分子生物学研究奠定基础。
研究運用SMART技術對藍點馬鮫頭腎構建瞭cDNA文庫，該文庫原始文庫滴度為2.26×106pfu/mL，文庫容量為1.36×106pfu，符閤文庫構建的要求。從文庫中隨機挑選24箇單剋隆菌，以載體的通用引物進行菌落檢測PCR，結果錶明：重組率為95.8%，插入片段大小平均為1000bp。該文庫的構建滿足瞭基因的分離與篩選，為進一步研究其相關基因剋隆及分子生物學研究奠定基礎。
연구운용SMART기술대람점마교두신구건료cDNA문고，해문고원시문고적도위2.26×106pfu/mL，문고용량위1.36×106pfu，부합문고구건적요구。종문고중수궤도선24개단극륭균，이재체적통용인물진행균락검측PCR，결과표명：중조솔위95.8%，삽입편단대소평균위1000bp。해문고적구건만족료기인적분리여사선，위진일보연구기상관기인극륭급분자생물학연구전정기출。
cDNA library of Scomberomorus niphonius head kidney was constracted by SMART technology. The original library titer is 2.26×106pfu/mL,and library capacity is 1.36×106pfu,meeting the requirements of library construction. RandomLy selected 24 monoclonal bacteria from the library,subjected to colony PCR detected by using universal primers. The results showed that recombination rate was 95.8%,an average in?sert size was 1 000bp. Construction of the library meet the isolation and screening of gene,and lay the foundation for further study of melecμlar biology and gene cloning.
