安徽农学通报
安徽農學通報
안휘농학통보
AUHUI AGRICULTURAL SCIENCE BULLETIN
2015年
17期
26-28,35
,共4页
HPLC%固相萃取%三叶青%槲皮素%山奈酚
HPLC%固相萃取%三葉青%槲皮素%山奈酚
HPLC%고상췌취%삼협청%곡피소%산내분
High performance liquid chromatography%Solid phase extraction%Radix tetrastigmae%Quercetin%Kaempferol
目的：建立HPLC法测定三叶青中槲皮素和山奈酚的方法。方法：三叶青组培物样品经甲醇超声提取，HLB固相萃取小柱净化，在色谱条件：色谱柱Varian ODS C18（4.6mm×250mm×5μm）、柱温30℃、流动相0.2%磷酸水-甲醇、流速1.0mL/min、检测波长360nm下进行测定。结果：槲皮素和山奈酚的方法检出限和线性范围分别为0.05mg/kg和0.05～50μg/mL，且线性范围内的相关系数均大于0.9999，加标回收率为71.9%～105.8%，相对标准偏差为1.5%～3.4%。结论：HPLC法结合固相萃取技术可用于测定三叶青中槲皮素和山奈酚的含量，方法检出限低，结果准确可靠，重复性好。
目的：建立HPLC法測定三葉青中槲皮素和山奈酚的方法。方法：三葉青組培物樣品經甲醇超聲提取，HLB固相萃取小柱淨化，在色譜條件：色譜柱Varian ODS C18（4.6mm×250mm×5μm）、柱溫30℃、流動相0.2%燐痠水-甲醇、流速1.0mL/min、檢測波長360nm下進行測定。結果：槲皮素和山奈酚的方法檢齣限和線性範圍分彆為0.05mg/kg和0.05～50μg/mL，且線性範圍內的相關繫數均大于0.9999，加標迴收率為71.9%～105.8%，相對標準偏差為1.5%～3.4%。結論：HPLC法結閤固相萃取技術可用于測定三葉青中槲皮素和山奈酚的含量，方法檢齣限低，結果準確可靠，重複性好。
목적：건립HPLC법측정삼협청중곡피소화산내분적방법。방법：삼협청조배물양품경갑순초성제취，HLB고상췌취소주정화，재색보조건：색보주Varian ODS C18（4.6mm×250mm×5μm）、주온30℃、류동상0.2%린산수-갑순、류속1.0mL/min、검측파장360nm하진행측정。결과：곡피소화산내분적방법검출한화선성범위분별위0.05mg/kg화0.05～50μg/mL，차선성범위내적상관계수균대우0.9999，가표회수솔위71.9%～105.8%，상대표준편차위1.5%～3.4%。결론：HPLC법결합고상췌취기술가용우측정삼협청중곡피소화산내분적함량，방법검출한저，결과준학가고，중복성호。
Objective:A high performance liquid chromatography method for determination of quercetin and kaempferol in Radix tetrastigmae was studied. Method:The samples of tissue cultures were extracted with methanol by ultrasonic and purified with HLB solid phase extraction. Then,the analysis was performed on a Varian ODS C18(4.6mm × 250mm × 5μm)chromatographic column with methanol and 0.2% phosphoric acid as mobile phase at a flow-rate of 1.0mL/min,the column temperature was 30℃ and the detection wave?length was 360nm.Results:method detection limits for quercetin and kaempferol were both 0.05mg/kg,and good linearity for them over the range of 0.05~50μg/mL(r>0.999 9). The average recovery and RSD of the method for quercetin and kaempferol were 71.9%~105.8% and 1.5%~3.4%,respectively. Conclusion:high performance liquid chromatography(HPLC)method combined with solid phase extraction technology can be used for the determination of quercetin and kaempferol in Radix tetrastigmae with low method detection limits,high accuracy and good repeatability.
