CAJ | 학술논문

背景：灯盏花素治疗脑梗死疗效确切、不良反应少、远期疗效稳定、毒副作用少，能够改善脑梗死后中枢神经系统受损区的微环境。 　　目的：探讨灯盏花素注射液联合骨髓间充质干细胞移植对脑梗死神经功能恢复及生长相关蛋白43表达的影响。 　　方法：将60只大脑中动脉闭塞模型 SD 大鼠随机分为脑梗死组、骨髓间充质干细胞移植组和联合组。建模6 h后通过尾静脉注射1 mL PBS、1 mL骨髓间充质干细胞悬液(2.5×106)、1 mL骨髓间充质干细胞悬液(2.5×106)+灯盏花素注射液75 mg/kg，连续注射5 d，1次/d。 　　结果与结论：移植后2周，免疫荧光法观察到 BrdU 阳性标记的骨髓间充质干细胞主要集中于梗死灶周围且联合组的BrdU阳性细胞数量多于骨髓间充质干细胞组及脑梗死组(P <0.01)；移植后1，2，3周联合组的神经功能障碍评分明显低于骨髓间充质干细胞组及脑梗死组(P <0.05)；移植后2周，与骨髓间充质干细胞组及脑梗死组比较，联合组的脑梗死面积明显减小，水肿程度明显减轻，生长相关蛋白43的表达明显增高(P <0.05)。光镜下联合组脑梗死组织中胶质细胞明显增生，脑组织水肿有明显减轻。结果表明灯盏花素注射液联合骨髓间充质干细胞可明显减轻脑梗死面积及水肿程度，促进大鼠脑梗死后神经功能恢复及梗死灶生长相关蛋白43的表达。
배경：등잔화소치료뇌경사료효학절、불량반응소、원기료효은정、독부작용소，능구개선뇌경사후중추신경계통수손구적미배경。 　　목적：탐토등잔화소주사액연합골수간충질간세포이식대뇌경사신경공능회복급생장상관단백43표체적영향。 　　방법：장60지대뇌중동맥폐새모형 SD 대서수궤분위뇌경사조、골수간충질간세포이식조화연합조。건모6 h후통과미정맥주사1 mL PBS、1 mL골수간충질간세포현액(2.5×106)、1 mL골수간충질간세포현액(2.5×106)+등잔화소주사액75 mg/kg，련속주사5 d，1차/d。 　　결과여결론：이식후2주，면역형광법관찰도 BrdU 양성표기적골수간충질간세포주요집중우경사조주위차연합조적BrdU양성세포수량다우골수간충질간세포조급뇌경사조(P <0.01)；이식후1，2，3주연합조적신경공능장애평분명현저우골수간충질간세포조급뇌경사조(P <0.05)；이식후2주，여골수간충질간세포조급뇌경사조비교，연합조적뇌경사면적명현감소，수종정도명현감경，생장상관단백43적표체명현증고(P <0.05)。광경하연합조뇌경사조직중효질세포명현증생，뇌조직수종유명현감경。결과표명등잔화소주사액연합골수간충질간세포가명현감경뇌경사면적급수종정도，촉진대서뇌경사후신경공능회복급경사조생장상관단백43적표체。
BACKGROUND:Breviscapine treatment of cerebral infarction has curative effects, few side effects, stable long-term efficacy and few side effects, which can improve the micro-environment of damaged central nervous system after cerebral infarction. OBJECTIVE:To investigate the effects of breviscapine injection combined with bone marrow mesenchymal stem cel transplantation on neurological recovery and growth-associated protein 43 expression in rats after cerebral infarction. METHODS:Sixty Sprague-Dawley rats undergoing middle cerebral artery occlusion were randomized into cerebral infarction group, cel transplantation group and combined group. At 6 hours after modeling, 1 mL PBS, 1 mL bone marrow mesenchymal stem cel suspension (2.5×106), and 1 mL bone marrow mesenchymal stem cel suspension (2.5×106)+75 mg/kg breviscapine injection were respectively injected via the tail vein in the three groups, once a day, continuously for 5 days. RESULTS AND CONCLUSION:At 2 weeks after transplantation, BrdU-positive bone marrow mesenchymal stem cels were mainly gathered in the peri-infarction region, and the number of positive cels was higher in the combined group than the other two groups (P < 0.01). At 1, 2, 3 weeks after transplantation, the neurological deficit scores were significantly lower in the combined group than the other two groups (P < 0.05). At 2 weeks after transplantation, the combined group had smaler infarct size, milder edema, and higher expression of growth-associated protein 43 as compared with the other two groups (P < 0.05). Under light microscope, glial cels proliferated dramaticaly and brain edema significantly reduced in the combined group. These findings indicate that breviscapine injection combined with bone marrow mesenchymal stem cel transplantation can significantly reduce infarct size and brain edema, promote neurological recovery and increase the expression of growth-associated protein 43 in rats with cerebral infarction.