CAJ | 학술논문

背景：随着医学模式的不断发展进步，人们对于骨缺损的治疗以及后期康复水平提出了更高的要求，这就使得以干细胞培养为基础的骨组织工程技术成为骨缺损治疗中较为重要的组成部分。 　　目的：探讨同种异体脂肪干细胞复合纳米级胶原基骨材料修复兔尺骨缺损的可行性。 　　方法：选取成年雌性新西兰兔70只作为研究对象，其中10只用于脂肪间充质干细胞的分离培养，60只采取随机数字表法分为单纯支架组、支架材料结合自体骨组、支架材料结合异体脂肪干细胞组，每组20只，均行尺骨中段部分切除，构建12 mm的骨缺损，然后植入相应的材料。植入材料6，9，12周测量骨密度，植入材料14周后观察3组坚固融合、非坚固融合、假关节形成比例。 　　结果与结论：支架材料结合异体脂肪干细胞组坚固融合比例显著高于单纯支架组、支架材料结合自体骨组，非坚固融合及假关节比例显著低于单纯支架组、支架材料加自体骨组(P <0.05)。单纯支架组坚固融合比例显著低于支架材料结合自体骨组，非坚固融合及假关节比例显著高于支架材料结合自体骨组(P <0.05)。支架材料结合异体脂肪干细胞组植入材料后第6，9，12周骨密度明显高于单纯支架组、支架材料结合自体骨组(P <0.05)。单纯支架组骨密度明显低于支架材料结合自体骨组(P <0.05)。结果表明同种异体脂肪干细胞复合纳米级胶原基骨材料修复尺骨缺损效果确切，骨密度较高。
배경：수착의학모식적불단발전진보，인문대우골결손적치료이급후기강복수평제출료경고적요구，저취사득이간세포배양위기출적골조직공정기술성위골결손치료중교위중요적조성부분。 　　목적：탐토동충이체지방간세포복합납미급효원기골재료수복토척골결손적가행성。 　　방법：선취성년자성신서란토70지작위연구대상，기중10지용우지방간충질간세포적분리배양，60지채취수궤수자표법분위단순지가조、지가재료결합자체골조、지가재료결합이체지방간세포조，매조20지，균행척골중단부분절제，구건12 mm적골결손，연후식입상응적재료。식입재료6，9，12주측량골밀도，식입재료14주후관찰3조견고융합、비견고융합、가관절형성비례。 　　결과여결론：지가재료결합이체지방간세포조견고융합비례현저고우단순지가조、지가재료결합자체골조，비견고융합급가관절비례현저저우단순지가조、지가재료가자체골조(P <0.05)。단순지가조견고융합비례현저저우지가재료결합자체골조，비견고융합급가관절비례현저고우지가재료결합자체골조(P <0.05)。지가재료결합이체지방간세포조식입재료후제6，9，12주골밀도명현고우단순지가조、지가재료결합자체골조(P <0.05)。단순지가조골밀도명현저우지가재료결합자체골조(P <0.05)。결과표명동충이체지방간세포복합납미급효원기골재료수복척골결손효과학절，골밀도교고。
BACKGROUND:With the continuous development of medical model, high-level treatment and rehabilitation for bone defects are required. Therefore, bone tissue engineering technique based on stem cel culture has become a more important component of bone defect repair. OBJECTIVE:To explore and analyze the feasibility of alogeneic adipose-derived stem cels combined with nano colagen-based bone for repair of ulna bone defects. METHODS:Seventy adult female New Zealand rabbits were selected, 10 of which were used to isolate and culture adipose-derived stem cels, another 60 were randomized into scaffold group, scaffold+bone autograft group, scaffold+alogeneic adipose-derived stem cels group, with 20 rabbits in each group. The middle part of the ulna was resected to construct 12-mm bone defect models in the three groups, and then, corresponding materials were implanted. At 6, 9 and 12 weeks after implantation, bone mineral density was detected; at 14 weeks after implantation, the rates of solid fusion rate, non-solid fusion, and false joint were observed. RESULTS AND CONCLUSION:Compared with the other two groups, the rate of solid fusion was significantly higher and the rates of non-solid fusion and false joint were lower in the scaffold+alogeneic adipose-derived stem cels group (P < 0.05). Compared with the scaffold group, the rate of solid fusion was higher and the rates of non-solid fusion and false joint were significantly lower in the scaffold+bone autograft group (P< 0.05). The bone mineral density increased dramaticaly at 6, 9, 12 weeks after implantation of scaffold+alogeneic adipose-derived stem cels, which was significantly higher than that in the scaffold group and scaffold+bone autograft group (P < 0.05). Compared with the scaffold group, the bone mineral density in the scaffold+bone autograft group was significantly higher (P < 0.05). These findings indicate that alogeneic adipose-derived stem cels combined with nano colagen-based bone has exact fusion effects with a higher bone mineral density.