中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2015年
34期
5428-5432
,共5页
生物材料%口腔生物材料%飞秒激光%纯钛%TiO2颗粒喷砂%细胞增殖%细胞分化
生物材料%口腔生物材料%飛秒激光%純鈦%TiO2顆粒噴砂%細胞增殖%細胞分化
생물재료%구강생물재료%비초격광%순태%TiO2과립분사%세포증식%세포분화
Titanium%Lasers%Surface Properties%Fibroblasts
背景：目前对种植体表面优化处理促进人工牙种植体骨整合的形成是近年来这一领域的研究热点，而通过飞秒激光这种表面处理钛金属表面改性促进成纤维细胞的增殖和分化的相关性研究报道较少。目的：观察飞秒激光处理对于人牙周膜成纤维细胞在钛金属表面增殖和分化的影响。方法：将人牙周膜成纤维细胞接种在分别经过飞秒激光处理、TiO2颗粒喷砂和砂纸顺向抛光的纯钛片表面(飞秒激光组，TiO2颗粒喷砂组和对照组)，观察细胞增殖和分化情况。结果与结论：与对照组相比，飞秒激光组和TiO2颗粒喷砂组钛片表面粗糙度明显增加，表面细胞密度明显增加，RUNX2和OSX mRNA表达水平增加；且飞秒激光组上述指标高于TiO2颗粒喷砂组。提示飞秒激光处理的纯钛表面更加粗糙，更有利于人牙周膜成纤维细胞的增殖和分化。
揹景：目前對種植體錶麵優化處理促進人工牙種植體骨整閤的形成是近年來這一領域的研究熱點，而通過飛秒激光這種錶麵處理鈦金屬錶麵改性促進成纖維細胞的增殖和分化的相關性研究報道較少。目的：觀察飛秒激光處理對于人牙週膜成纖維細胞在鈦金屬錶麵增殖和分化的影響。方法：將人牙週膜成纖維細胞接種在分彆經過飛秒激光處理、TiO2顆粒噴砂和砂紙順嚮拋光的純鈦片錶麵(飛秒激光組，TiO2顆粒噴砂組和對照組)，觀察細胞增殖和分化情況。結果與結論：與對照組相比，飛秒激光組和TiO2顆粒噴砂組鈦片錶麵粗糙度明顯增加，錶麵細胞密度明顯增加，RUNX2和OSX mRNA錶達水平增加；且飛秒激光組上述指標高于TiO2顆粒噴砂組。提示飛秒激光處理的純鈦錶麵更加粗糙，更有利于人牙週膜成纖維細胞的增殖和分化。
배경：목전대충식체표면우화처리촉진인공아충식체골정합적형성시근년래저일영역적연구열점，이통과비초격광저충표면처리태금속표면개성촉진성섬유세포적증식화분화적상관성연구보도교소。목적：관찰비초격광처리대우인아주막성섬유세포재태금속표면증식화분화적영향。방법：장인아주막성섬유세포접충재분별경과비초격광처리、TiO2과립분사화사지순향포광적순태편표면(비초격광조，TiO2과립분사조화대조조)，관찰세포증식화분화정황。결과여결론：여대조조상비，비초격광조화TiO2과립분사조태편표면조조도명현증가，표면세포밀도명현증가，RUNX2화OSX mRNA표체수평증가；차비초격광조상술지표고우TiO2과립분사조。제시비초격광처리적순태표면경가조조，경유리우인아주막성섬유세포적증식화분화。
BACKGROUND:Currently, the implant surface is optimized to promote the osseointegration of artificial dental implants, which has become a research focus in recent years. But the femtosecond laser is less reported to modify the titanium surface so as to promote fibroblast proliferation and differentiation. OBJECTIVE: To observe the effect of femtosecond laser on the proliferation and differentiation of human periodontal ligament fibroblasts on titanium surface. METHODS: Human periodontal ligament fibroblasts were respectively inoculated on different titanium surfaces of the three groups (femtosecond laser group, TiO2 particle blasted group and pure titanium group). Then differentiation and proliferation of human periodontal ligament fibroblasts were observed. RESULTS AND CONCLUSION:There was a significant difference in the surface roughness among these three groups, and compared with the pure titanium group, the attached cels were more in the femtosecond laser group and TiO2 particle blasted group. mRNA expressions of RUNX2 and OSX were highest in the femtosecond laser group folowed by TiO2 particle blasted group and pure titanium group in order. These findings indicate that pure titanium treated with femtosecond laser has fine biological property and may be more feasible for human periodontal ligament fibroblasts to attach, grow and proliferate.
